Cell dyn 3700 and 4000

A complete clinical analysis -consisting of hematology, chemistry, and urine chemistry analysis -was performed at the onset of and after the experimental period. All samples (blood and urine) were collected by a nurse from the subjects early in the morning, under fasting conditions. Blood samples at rest were obtained by venipuncture, at the beginning and at the end experimental period and were placed in different tubes according to the analytical procedures. Samples were processed within 1 h of collection and stored at -80ºC for the analytical determinations. The hematological parameters were recorded using an automated hematological analyzer (CellDyn 3700 and 4000, Abbott, IL, USA) at the Clinical Analysis Service of the Hospital Virgen de la Arrixaca (Murcia, Spain).
Twenty-four-hour urine samples were collected before and after the 2-weeks training period. They were collected in sterile, dark polystyrene tubes with screw caps. The urine analyses were also performed in a modular analyzer (Roche Diagnostic, Mannheim, Germany). The 24-h -1 urine was used for the absolute calculation of the amounts of F 4 -NeuroPs and F 2 -dihomo-IsoPs excreted. The urinary F 4 -NeuroPs and F 2 -dihomo-IsoPs were analyzed using our previously-described method [27] .