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Recombinant murine interleukin 4 il 4

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Recombinant murine interleukin-4 (IL-4) is a cytokine produced by activated T cells, mast cells, and basophils. It plays a central role in the regulation of immune and inflammatory responses.

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Lab products found in correlation

Biotin conjugated goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Rgdsp and rgesp peptides, by Bachem (2 mentions) Horseradish peroxidase conjugated anti mouse and anti rabbit secondary antibodies, by Cytiva (2 mentions) Biotin conjugated goat anti rat igg antibody, by Thermo Fisher Scientific (2 mentions) Hrp conjugated streptavidin, by Thermo Fisher Scientific (2 mentions)

2 protocols using recombinant murine interleukin 4 il 4

1

Recombinant CCN1 Proteins Production

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Recombinant CCN1 and mutant proteins (D125A, DM, CCN1-I.II, CCN1-ΔCT, and D125A-ΔCT) were produced using a baculovirus expression system in insect cells and purified by ion-exchange or immuno-affinity chromatography30 (link), 32 (link). Rabbit polyclonal antibodies against CCN1 were raised against the CCN1 vWC domain67 (link). Rabbit and mouse IgGs, echstatin, and lipopolysacharide (LPS) were from Sigma. RGDSP and RGESP peptides were from Bachem. Function-blocking mAbs against mouse integrin β1 (KM16), αv (RMV-7), β3 (2C9-G3), αvβ3 (23-C6) and β5 (KN52) were from eBioscience. Recombinant murine interleukin-4 (IL-4) were from R&D systems. Horse radish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were from Amersham Biosciences. Biotin-conjugated goat anti-rabbit IgG, biotin-conjugated goat anti-rat IgG antibody, and streptavidin-conjugated HRP were from Invitrogen.
Jun J.I., Kim K.H, & Lau L.F. (2015). The Matricellular Protein CCN1 Mediates Neutrophil Efferocytosis in Cutaneous Wound Healing. Nature communications, 6, 7386.
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2

Recombinant CCN1 Proteins Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant CCN1 and mutant proteins (D125A, DM, CCN1-I.II, CCN1-ΔCT, and D125A-ΔCT) were produced using a baculovirus expression system in insect cells and purified by ion-exchange or immuno-affinity chromatography30 (link), 32 (link). Rabbit polyclonal antibodies against CCN1 were raised against the CCN1 vWC domain67 (link). Rabbit and mouse IgGs, echstatin, and lipopolysacharide (LPS) were from Sigma. RGDSP and RGESP peptides were from Bachem. Function-blocking mAbs against mouse integrin β1 (KM16), αv (RMV-7), β3 (2C9-G3), αvβ3 (23-C6) and β5 (KN52) were from eBioscience. Recombinant murine interleukin-4 (IL-4) were from R&D systems. Horse radish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were from Amersham Biosciences. Biotin-conjugated goat anti-rabbit IgG, biotin-conjugated goat anti-rat IgG antibody, and streptavidin-conjugated HRP were from Invitrogen.
Jun J.I., Kim K.H, & Lau L.F. (2015). The Matricellular Protein CCN1 Mediates Neutrophil Efferocytosis in Cutaneous Wound Healing. Nature communications, 6, 7386.
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