Chitosan (CS: Molecule weight: 100,000-300,000 Da, practical amine: 7-12%) was purchased from Polysciences, Inc., USA. polyethylene oxide (PEO, Molecule weight: 400,000 Da), glucose oxidase (GOx, from Aspergillus niger, 50KU), glutaraldehyde (GA, 25% aqueous solution) and metronidazole (MNZ) were all purchased from Sigma-Aldrich, USA. Osteoblasts used in cells culture study were derived from rats. For antibacterial activity, P. gingivalis ATCC 33277 was grown in Trypticase soy broth (BBL™, BD Company, USA), supplemented with 0.1g (per 100 ml) yeast extract (BD Company, USA), 0.5 mg hemin (Sigma-Aldrich, USA) and 0.1 mg vitamin K (Menadione, Sigma-Aldrich, USA).
Metronidazole mnz
Manufactured by Merck Group
Sourced in United States
Metronidazole (MNZ) is a pharmaceutical compound used as a laboratory reagent. It is a white to pale yellow crystalline powder with the molecular formula C₆H₉N₃O₃. Metronidazole is a synthetic antimicrobial agent that exhibits activity against certain anaerobic bacteria and protozoa.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by BD (1 mentions)
Hemin, by Merck Group (1 mentions)
Vitamin k menadione, by Merck Group (1 mentions)
Polyethylene oxide peo, by Merck Group (1 mentions)
Nodinitib 1, by MedChemExpress (1 mentions)
A100382, by Sangon (1 mentions)
Ampicillin, by Sangon (1 mentions)
Vancomycin, by Sangon (1 mentions)
Streptomycin, by Sangon (1 mentions)
2 protocols using metronidazole mnz
1
Chitosan-PEO Hydrogel for Osteoblast Culture
2
NOD1 Inhibitor Evaluation: In Vitro and In Vivo
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DTX was purchased from Jiangsu Hengrui Medicine Company (H20020543, Jiangsu, China) and diluted to the desired concentrations freshly before each experiment. Concentrations and durations of treatment were indicated in the corresponding legends. The commercial NOD1 inhibitor Nodinitib-1 (HY-18639, MedChemExpress) was used at 5–10 μmol/L for 48–72 h in vitro and 20 mg/kg in vivo. Metronidazole (MNZ) was purchased from Sigma–Aldrich (M3761). All antibiotics (Ampicillin, Streptomycin, Vancomycin, and Colistin) were purchased from Sangon Biotech (A100339, A100382, A100990, A610318). Lysosome trackerTM Deep Red was purchased from Thermo Fisher Scientific, Inc. (L12492). The HIS-BFT-1 and FLAG-NOD1 protein were custom-designed from Zoonbio Biotechnology (Nanjing, China). Human Recombinant Protein GAK (NM_005255) and NUMB (NM_001005743) were purchased from ORIGENG.
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