Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
Mouse anti human rhoa
Manufactured by Santa Cruz Biotechnology
Mouse anti–human RhoA is a primary antibody that specifically recognizes the RhoA protein, a small GTPase involved in the regulation of the actin cytoskeleton. This antibody can be used to detect the expression and localization of RhoA in human samples.
Automatically generated - may contain errors
Lab products found in correlation
Iseq00010, by Merck Group (2 mentions)
Ab64771, by Abcam (2 mentions)
Ab2386, by ABclonal (2 mentions)
Mouse anti human collagen type 1, by Proteintech (2 mentions)
Rabbit anti human tfrc, by ZenBio (2 mentions)
Mouse anti human iga antibody, by Southern Biotech (2 mentions)
Rabbit anti human β tubulin, by ZenBio (2 mentions)
Ecl plus detecting system, by GE Healthcare (2 mentions)
Rabbit anti human gapdh, by Bioworld Technology (2 mentions)
2 protocols using mouse anti human rhoa
1
Protein Expression Analysis by Western Blot
2
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
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