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Citrate synthase kit

Manufactured by Merck Group
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Sourced in United States

The Citrate synthase kit is a laboratory tool designed to measure the activity of the citrate synthase enzyme. Citrate synthase is a key enzyme in the citric acid cycle, a crucial metabolic pathway that occurs in the mitochondria of cells. The kit provides the necessary reagents and protocols to quantify citrate synthase activity, which can be used to assess mitochondrial function and energy metabolism in various biological samples.

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Lab products found in correlation

Nad nadh assay kit, by BioAssay Systems (1 mentions) Qproteome mitochondria isolation kit, by Qiagen (1 mentions)

Close spelling variants of this product

Citrate Synthase Activity Kit Citrate Synthase Activity Assay Kit Citrate Synthase Assay Kit Citrate synthase

2 protocols using citrate synthase kit

1

Citrate Synthase Activity Quantification

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CS activity was assessed using the citrate synthase kit (Sigma-Aldrich, St. Louis, MO, USA). CS activity in muscle was expressed as micromole per min per μg of protein.
Hsu M.F., Yu S.H., Korivi M., Jean W.H., Lee S.D., Huang C.Y., Liao Y.H., Lu J, & Kuo C.H. (2017). Hormetic Property of Ginseng Steroids on Anti-Oxidant Status against Exercise Challenge in Rat Skeletal Muscle. Antioxidants, 6(2), 36.
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2

Mitochondrial Enzyme and Cofactor Assays

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Citrate synthase (EC Number: 2.3.3.1) activity in isolated mitochondria preparations of epididymal WAT was measured with spectrophotometer using citrate synthase kit (Sigma-Aldrich) following manufacturer's instructions. NAD+ and NADH levels in isolated mitochondria preparations were determined using the NAD/NADH assay kit purchased from BioAssay Systems (Hayward, CA, USA). Mitochondria were isolated using the Qproteome Mitochondria Isolation Kit (Qiagen).
Yu J., Yu B., He J., Zheng P., Mao X., Han G, & Chen D. (2014). Chronic Glucocorticoid Exposure-Induced Epididymal Adiposity Is Associated with Mitochondrial Dysfunction in White Adipose Tissue of Male C57BL/6J Mice. PLoS ONE, 9(11), e112628.
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