One microgram total RNA collected from rat small intestine was subjected to Illumina TruSeq RNA library construction. The sequencing libraries were barcoded and sequencing reaction was performed using Illumina HiSeq 2500 in National Yang-Ming University Genome Research Center. Raw sequence data was aligned to Rnor_6.0 genome with Ensembl release-85 gene annotation using TopHat v2.1.1 [30 (link), 31 (link)]. The aligned reads were assembled according to Ensembl gene and transcript annotation using Cufflinks v2.2.1 [32 (link)], and the gene level read count matrix was loaded into DESeq2 [33 (link)] and Wald statistical testing was performed. Differential expressed genes with Benjamini-Hochberg false discovery rate (FDR) less than 0.05 and fold-change greater than 1.5 were considered significant. The flowchart of study design was demonstrated at Suppl. Fig. 1 . The rat samples are listed in Table 1 .
Truseq rna library construction
Manufactured by Illumina
The TruSeq RNA library construction is a lab equipment product designed for preparing RNA samples for sequencing. It provides a standardized workflow for constructing libraries from total RNA or poly-A selected RNA. The core function of this product is to enable the generation of high-quality sequencing libraries from RNA samples.
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Lab products found in correlation
2 protocols using truseq rna library construction
1
Rat Small Intestine Transcriptome Analysis
2
Transcriptome Analysis of Rat Gastric Tissues
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Total RNA collected from gastric tissues of rats in each group (n = 6 per group) was subjected to Illumina TruSeq RNA library construction. The sequencing libraries were barcoded and the sequencing reaction was performed using Illumina NovaSeq 6000. A cut-off of 2-fold change and p < 0.05 were used to define the threshold of significance, identifying differentially expressed genes. The R programming language was used for Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
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