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Xenolight rediject d luciferin ultra

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XenoLight RediJect D-Luciferin Ultra is a bioluminescent substrate used in in vivo imaging applications. It is designed to provide high light output for sensitive detection of luciferase-expressing cells and tissues.

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XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate XenoLight® RediJect™ D-Luciferin RediJect D-Luciferin Ultra RediJect D-Luciferin XenoLight D-Luciferin D-luciferin Luciferin

2 protocols using xenolight rediject d luciferin ultra

1

In Vivo Imaging of Adenovirus Vectors

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Mice were initially shaved using Nair™hair removal (Church & Dwight Co). Animals were immunized intramuscularly (quadriceps) with 1010 vp of Ad5 or Ad26 expressing luciferase. In the selected time-points 3, 6, 12, 24 hours and 21 days animals were injected i.p. with 150 μl (150 mg/Kg) of XenoLight RediJect D-Luciferin Ultra (PerkinElmer). After 10 minutes, imaging was performed using the IVIS Series 100 (Xenogen) with an exposure time of 2 minutes. Images and luminescence measurements were performed using Living Image software (version 2.50.1; Xenogen) (47 (link)).
Larocca R.A., Provine N.M., Aid M., Iampietro M.J., Borducchi E.N., Badamchi-Zadeh A., Abbink P., Ng’ang’a D., Bricault C.A., Blass E., Penaloza-MacMaster P., Stephenson K.E, & Barouch D.H. (2016). Adenovirus serotype 5 vaccine vectors trigger IL-27-dependent inhibitory CD4+ T cell responses that impair CD8+ T cell function. Science immunology, 1(5), eaaf7643.
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2

In Vivo Bioluminescence Imaging

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Luciferase transgene expression through luminescence was quantified using an IVIS Series 100 imaging device and Living Image software v2.50.1 (Xenogen). At selected time points, mice were anesthetized by 1.5% isoflurane inhalation and injected i.p. with 150 μl of XenoLight RediJect d-luciferin Ultra (150 mg/kg of body weight; PerkinElmer). Imaging was performed for a 60-s exposure time, 1.2 f/stop, and medium binning.
Badamchi-Zadeh A., Tartaglia L.J., Abbink P., Bricault C.A., Liu P.T., Boyd M., Kirilova M., Mercado N.B., Nanayakkara O.S., Vrbanac V.D., Tager A.M., Larocca R.A., Seaman M.S, & Barouch D.H. (2018). Therapeutic Efficacy of Vectored PGT121 Gene Delivery in HIV-1-Infected Humanized Mice. Journal of Virology, 92(7), e01925-17.
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