Ha fragments

Manufactured by Lifecore Biomedical

HA fragments are a type of lab equipment produced by Lifecore Biomedical. They are composed of hyaluronic acid, a naturally occurring polysaccharide. The core function of HA fragments is to provide a source of hyaluronic acid for various applications in research and development.

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3 protocols using ha fragments

Adipogenic Effects of HA Fragments

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Three different molecular weight HA fragments were chosen: 41–65 kDa, 151–300 kDa and 1.01–1.8 MDa (LifeCore Biomedical, LLC. MN, USA). For convenience, these will be referred to as 65 kDa, 300 kDa and 1 MDa respectively. The HA powder was dissolved in DMEM/F12 and further diluted in ADM to the desired concentration (100 and 200 μg/mL). On Day 0, cells were treated with HA fragments in ADM and replaced every 3 days until the end of adipogenesis.

Wilson N., Steadman R., Muller I., Draman M., Rees D.A., Taylor P., Dayan C.M., Ludgate M, & Zhang L. (2019). Role of Hyaluronan in Human Adipogenesis: Evidence from in-Vitro and in-Vivo Studies. International Journal of Molecular Sciences, 20(11), 2675.

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HA Preparation for In Vivo and In Vitro Studies

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Highly purified, certified endotoxin free, HA fragments were purchased from Lifecore Biomedical, LLC and were used for all experiments. For in vivo studies, HA35 was dissolved at 2 mg/ml in the drinking water provided in our animal housing facility and dissolved with shaking at 4 °C overnight before being aliquoted and frozen in −80 °C until use. For in vitro studies, HA was dissolved in sterile Milli Q purified water (HA35 at 5 mg/ml and HA2000 was dissolved at 2.5 mg/ml, to prevent gel formation).

Kim Y., West G.A., Ray G., Kessler S.P., Petrey A.C., Fiocchi C., McDonald C., Longworth M.S., Nagy L.E, & de la Motte C.A. (2017). Layilin is critical for mediating hyaluronan 35 kDa-induced intestinal epithelial tight junction protein ZO-1 in vitro and in vivo. Matrix biology : journal of the International Society for Matrix Biology, 66, 93-109.

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Hyaluronic Acid Fragmentation in Drinking Water

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Commercially available highly purified HA fragments (Lifecore Biomedical, LLC) were dissolved at 1.2mg/ml in the drinking water provided in our animal housing facility and kept on a shaker at 4°C overnight before being aliquoted and frozen in −80°C until use.

Kim Y., Kessler S.P., Obery D.R., Homer C.R., McDonald C, & de la Motte C.A. (2016). Hyaluronan 35 kDa treatment protects mice from Citrobacter rodentium infection and induces epithelial tight junction protein ZO-1 in vivo. Matrix biology : journal of the International Society for Matrix Biology, 62, 28-39.

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