Calnexin c5c9 rabbit monoclonal antibody

HEK 293T cells (ATCC CRL-3216 from American Type Culture Collection, Rockville, MD) and HEK 293T Lenti-X (Clontech/TaKaRa Bio) were cultured at 37°C in 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS; Gibco), 2 mM l-glutamine (Life Technologies), and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin; Life Technologies).
The following reagents and their commercial sources were used: tunicamycin and cycloheximide (Sigma), Kifunensine (Tocris Bioscience), ACE2 activity kit (no. K897, BioVision), anti-FLAG-agarose beads (Sigma), and 3× FLAG peptide (Sigma). The following antibodies were purchased from Thermo Fischer: SARS-CoV/SARS-CoV-2 spike protein S2 monoclonal antibody (1A9), protein disulfide-isomerase (PDI) mouse antibody (clone: RL90), and Alexa 594- and Alexa 488-conjugated antibodies against mouse and rabbit IgG, respectively. The monoclonal anti-FLAG M2 antibody was purchased from Sigma. The anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; D4C6R) monoclonal antibody, the golgin-97 (D8P2K) rabbit monoclonal antibody, the calnexin (C5C9) rabbit monoclonal antibody, and the FLAG tag (D6W5B) rabbit monoclonal antibody were from Cell Signaling. The human ACE2 Alexa Fluor 488-conjugated antibody (535919) was purchased from R&D systems.

HEK 293T cells (ATCC CRL-3216 from American Type Culture Collection, Rockville, MD) and HEK 293T Lenti-X (Clontech/TaKaRa Bio) were cultured at 37°C in 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS; Gibco), 2 mM l-glutamine (Life Technologies), and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin; Life Technologies).
The following reagents and their commercial sources were used: tunicamycin and cycloheximide (Sigma), Kifunensine (Tocris Bioscience), ACE2 activity kit (no. K897, BioVision), anti-FLAG-agarose beads (Sigma), and 3× FLAG peptide (Sigma). The following antibodies were purchased from Thermo Fischer: SARS-CoV/SARS-CoV-2 spike protein S2 monoclonal antibody (1A9), protein disulfide-isomerase (PDI) mouse antibody (clone: RL90), and Alexa 594- and Alexa 488-conjugated antibodies against mouse and rabbit IgG, respectively. The monoclonal anti-FLAG M2 antibody was purchased from Sigma. The anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; D4C6R) monoclonal antibody, the golgin-97 (D8P2K) rabbit monoclonal antibody, the calnexin (C5C9) rabbit monoclonal antibody, and the FLAG tag (D6W5B) rabbit monoclonal antibody were from Cell Signaling. The human ACE2 Alexa Fluor 488-conjugated antibody (535919) was purchased from R&D systems.