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Control mouse igg2a c1.18.4

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Control mouse IgG2a (C1.18.4) is a laboratory reagent used as a control in various immunological assays. It is a non-specific mouse immunoglobulin G2a (IgG2a) isotype.

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Lab products found in correlation

Normal rabbit serum, by Fujifilm (2 mentions)

2 protocols using control mouse igg2a c1.18.4

1

Selective Depletion of NK Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One day before infection, selective depletion16 (link) of NK cells was attained through a single intraperitoneal (i.p.) injection of 25 μg/mouse anti-NK1.1 monoclonal antibody (PK136) or 25 μg/mouse of a control mouse IgG2a (C1.18.4) produced by Bio-X-Cell (West Lebanon, NH). Alternatively, NK Cells were depleted through a single i.p. injection with 20 μL of anti-asialo GM1 (Wako Biochemicals, Richmond, VA) diluted in 180 μL of Hank’s balanced salt solution (HBSS) or 20 μL of normal rabbit serum (Wako Pure Chemical Industries, Japan) diluted in 180 μL HBSS.
Rydyznski C., Daniels K.A., Karmele E.P., Brooks T.R., Mahl S.E., Moran M.T., Li C., Sutiwisesak R., Welsh R.M, & Waggoner S.N. (2015). Generation of cellular immune memory and B-cell immunity are impaired by natural killer cells. Nature communications, 6, 6375.
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2

Selective Depletion of NK Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One day before infection, selective depletion16 (link) of NK cells was attained through a single intraperitoneal (i.p.) injection of 25 μg/mouse anti-NK1.1 monoclonal antibody (PK136) or 25 μg/mouse of a control mouse IgG2a (C1.18.4) produced by Bio-X-Cell (West Lebanon, NH). Alternatively, NK Cells were depleted through a single i.p. injection with 20 μL of anti-asialo GM1 (Wako Biochemicals, Richmond, VA) diluted in 180 μL of Hank’s balanced salt solution (HBSS) or 20 μL of normal rabbit serum (Wako Pure Chemical Industries, Japan) diluted in 180 μL HBSS.
Rydyznski C., Daniels K.A., Karmele E.P., Brooks T.R., Mahl S.E., Moran M.T., Li C., Sutiwisesak R., Welsh R.M, & Waggoner S.N. (2015). Generation of cellular immune memory and B-cell immunity are impaired by natural killer cells. Nature communications, 6, 6375.
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