The protein contents of PMNs and MNs were determined using the bicinchoninic acid protein (BCA) assay kit protocol (Sigma-Aldrich, Madrid, Spain) according to the manufacturer´s instructions.
Bca assay kit protocol
Manufactured by Merck Group
Sourced in Spain
The BCA (Bicinchoninic Acid) Assay Kit is a colorimetric assay protocol used for the quantitative determination of total protein concentration. The kit utilizes the BCA reagent, which reacts with proteins to produce a purple-colored complex that absorbs light at 562 nm. This provides a simple and accurate method for measuring protein levels in a variety of samples.
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Lab products found in correlation
2 protocols using bca assay kit protocol
1
Protein Quantification in PMNs and MNs
2
BCA Assay for Protein Quantification
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Protein concentration of the samples was measured following BCA assay kit protocol (Sigma-Aldrich).
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