Cervical cancer cell lines (SiHa, CaSki, ME180, SW756, C33A and HT3) were obtained from the American Type Culture Collection (ATCC) and maintained in IMDM media (Life Technologies) with 10% heat-inactivated FBS. Normal cervix epithelial cell lines Ect1/E6E7 and End1E6/E7 maintained in Keratinocyte-Serum Free medium (GIBCO-BRL 17005–042) with 0.1 ng/ml human recombinant EGF and 0.05 mg/ml bovine pituitary extract. HCvEpC were obtained from Cell Applications, Inc and maintained in HCvEpC media. Experiments were performed on cell lines under passage 30. Short Tandem Repeat (STR) profiling was last performed November 2020 which confirmed positive match and full cell line authentication per ATCC reference standards. Mycoplasma testing was performed every 3 months to verify no contamination. Sodium oleate, linoleic acid, protease inhibitor, phosphatase inhibitor cocktails, PFTα, GW9662 and lipid mixture (L0288) were purchased from Sigma. Arachidonic acid was purchased from Cayman chemicals. Adipocyte conditioned media was purchased from ZenBio Inc (CMA-10).
Muhammad N., Ruiz F., Stanley J., Rashmi R., Cho K., Jayachandran K., Zahner M.C., Huang Y., Zhang J., Markovina S., Patti G.J, & Schwarz J.K. (2022). Mono- and diunsaturated fatty acids sensitize cervical cancer to radiation therapy. Cancer research, 82(24), 4515-4527.
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