The SuperScript® III First-Strand Synthesis System and AccuPrime™ Taq DNA Polymerase System were purchased from Invitrogen/Life Technologies Corporation, USA. Anti-TRPM7 (cat#ab85016), anti-Cofilin (phospho S3) antibody (cat#ab12866) and anti-Cofilin antibody [EP6376] (cat#ab134963) were purchased from Abcam, USA. Diamidino-2-phenylindole (DAPI, cat#4083), MMP-2 (D8N9Y) rabbit mAb (cat#13132), cleaved caspase-3 (Asp175) antibody (cat#9661), phosphor-Akt (ser473) antibody (p-Akt, cat#9271), Akt antibody (t-Akt, cat#9272) and phospho-p44/42 MAPK antibody (p-ERK1/2, cat#5726) were purchased from Cell Signaling Technology, USA. Anti-MAP Kinase ERK1/ERK2 Rabbit pAb (t-ERK1/2, cat#442704) was purchased from Millipore. Pierce™ BCA Protein Assay Kit was product of Pierce Biotechnology, USA. All cell culture materials were purchased from Gibco Life Technologies Corporation (USA). Sodium Dodecyl Sulfate (SDS), sodium chloride (NaCl) and phenylmethylsulfonyl fluoride (PMSF) were purchased from Bioshop, Canada. All other reagents used were obtained from Sigma-Aldrich, USA unless mentioned otherwise.
Mmp 2 d8n9y rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
MMP-2 (D8N9Y) rabbit mAb is a laboratory reagent used to detect and study the matrix metalloproteinase-2 (MMP-2) protein in biological samples. MMP-2 is an enzyme involved in the breakdown of extracellular matrix components. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions)
Accuprime taq dna polymerase system, by Thermo Fisher Scientific (1 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (1 mentions)
Anti trpm7, by Abcam (1 mentions)
Diamidino 2 phenylindole dapi, by Cell Signaling Technology (1 mentions)
Ecl system, by Cytiva (1 mentions)
Mmp 9 d6o3h xp rabbit mab, by Cell Signaling Technology (1 mentions)
β actin monoclonal antibody ab6276, by Abcam (1 mentions)
2 protocols using mmp 2 d8n9y rabbit mab
1
Western Blot Analysis of Signaling Proteins
2
Protein Expression Profiling in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was isolated and an equal quantity of protein lysates (50 g/well) were performed by SDS‐polyacrylamide gel electrophoresis, then protein was electrotransferred onto polyvinylidene difluoride membranes. The membrane was incubated with a blocking solution (TBS, 0.1% Tween 20 and 5% nonfat dried milk powder) to block nonspecific protein for 2 hour at room temperature. Primary antibody of TGFBI (D31B8) XP Rabbit mAb, MMP‐2 (D8N9Y) Rabbit mAb and MMP‐9 (D6O3H) XP Rabbit mAb were purchased from Cell Signaling Technology. Calpain 2 antibody (ab39165) and β‐actin monoclonal antibody (ab6276) were purchased from Abcam. The blots were incubated with antibodies overnight at room temperature, and the immune complex was detected using a peroxidase‐conjugated secondary antibody and ECL system (Amersham) according to the manufacturer's instructions.
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