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6 15n2 l lysine hydrochloride

Manufactured by Cambridge Isotopes

6,15N2 L-Lysine-hydrochloride is a stable isotope-labeled amino acid product. It is designed for use in research and analytical applications that require the incorporation of stable isotopes.

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2 protocols using 6 15n2 l lysine hydrochloride

1

SILAC Cell Culture Labeling Protocol

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For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), 1% penicillin/streptomycin, L-Arginine hydrochloride (85mg/ml Sigma) and either “light” L-Lysine hydrochloride (50μg/ml Sigma) or heavy 13C6,15N2 L-Lysine-hydrochloride (50mg/ml Cambridge Isotopes) and 292 mg/mL L-Glutamine (Mediatech).
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2

SILAC Cell Culture Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), 1% penicillin/streptomycin, L-Arginine hydrochloride (85mg/ml Sigma) and either “light” L-Lysine hydrochloride (50μg/ml Sigma) or heavy 13C6,15N2 L-Lysine-hydrochloride (50mg/ml Cambridge Isotopes) and 292 mg/mL L-Glutamine (Mediatech).
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