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Anti alkaline phosphatase monoclonal antibody clone 8b6

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The Anti-alkaline phosphatase monoclonal antibody (clone 8B6) is a laboratory reagent used for the detection and quantification of alkaline phosphatase in various biological samples. It is a highly specific antibody that binds to the alkaline phosphatase enzyme, enabling its identification and measurement through immunoassay techniques.

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Lab products found in correlation

Halt phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Complete edta free inhibitor cocktail, by Roche (2 mentions) Interferin transfection reagent, by Polyplus Transfection (2 mentions) Actin mab1501r, by Merck Group (2 mentions) Geneticin 1 10 phenanthroline monohydrate, by Merck Group (2 mentions) Pacs 2 sirna, by Qiagen (2 mentions) Pacs 2 antibody 19508 1 ap, by Proteintech (2 mentions) Pegfr ab40815, by Abcam (2 mentions) Egfr 06 847, by Merck Group (2 mentions) Adam9 af949, by R&D Systems (2 mentions) Adam17 mab9301, by R&D Systems (2 mentions) Green fluorescent protein expression plasmid, by Takara Bio (2 mentions)

Close spelling variants of this product

Alkaline phosphatase (284 citations) Phosphatases (7 citations)

2 protocols using anti alkaline phosphatase monoclonal antibody clone 8b6

1

PACS-2 Regulation of ADAM17-Mediated EGFR Signaling

Cited 1 time
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PMA and BB-94 were from Calbiochem. Geneticin, 1,10-phenanthroline monohydrate, MISSION Universal Negative Control siRNA #1, and anti-alkaline phosphatase monoclonal antibody (clone 8B6) were from Sigma-Aldrich. HALT Phosphatase Inhibitor Cocktail was from Thermo Scientific. Complete EDTA-free inhibitor cocktail was from Roche. Interferin transfection reagent was from PolyPlus, and siGENOME non-targeting siRNA pool #2, SMARTpool PACS-2 and ADAM17 siRNAs were from Dharmacon (Thermo Scientific). We additionally used PACS-2 siRNA from Qiagen and pooled three siRNAs (PACS2_6, PACS2_7 and PACS2_9). Recombinant murine TNF-α was from Strathmann Biotec AG. PACS-2 antibody (19508-1-AP) was from Proteintech. ADAM17 (ab39162), ADAM17 (ab2051), MT1-MMP (ab38971), ADAM10 (ab1997), and pEGFR (ab40815) antibodies were from Abcam. PACS-1 antibody 17703 and PACS-2 antibody 18193 were previously described53 (link),54 (link). Actin (MAB1501R) and EGFR (06–847) antibodies were from Millipore. ADAM9 (AF949), and ADAM17 (MAB9301) antibodies were from R&D Systems. PACS-2-HA expression plasmid was in a pcDNA.3 backbone40 (link) (Invitrogen), and green fluorescent protein (GFP) expression plasmid was from Clontech.
Dombernowsky S.L., Samsøe-Petersen J., Petersen C.H., Instrell R., Hedegaard A.M., Thomas L., Atkins K.M., Auclair S., Albrechtsen R., Mygind K.J., Fröhlich C., Howell M., Parker P., Thomas G, & Kveiborg M. (2015). The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17. Nature communications, 6, 7518.
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2

PACS-2 Regulation of ADAM17-Mediated EGFR Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PMA and BB-94 were from Calbiochem. Geneticin, 1,10-phenanthroline monohydrate, MISSION Universal Negative Control siRNA #1, and anti-alkaline phosphatase monoclonal antibody (clone 8B6) were from Sigma-Aldrich. HALT Phosphatase Inhibitor Cocktail was from Thermo Scientific. Complete EDTA-free inhibitor cocktail was from Roche. Interferin transfection reagent was from PolyPlus, and siGENOME non-targeting siRNA pool #2, SMARTpool PACS-2 and ADAM17 siRNAs were from Dharmacon (Thermo Scientific). We additionally used PACS-2 siRNA from Qiagen and pooled three siRNAs (PACS2_6, PACS2_7 and PACS2_9). Recombinant murine TNF-α was from Strathmann Biotec AG. PACS-2 antibody (19508-1-AP) was from Proteintech. ADAM17 (ab39162), ADAM17 (ab2051), MT1-MMP (ab38971), ADAM10 (ab1997), and pEGFR (ab40815) antibodies were from Abcam. PACS-1 antibody 17703 and PACS-2 antibody 18193 were previously described53 (link),54 (link). Actin (MAB1501R) and EGFR (06–847) antibodies were from Millipore. ADAM9 (AF949), and ADAM17 (MAB9301) antibodies were from R&D Systems. PACS-2-HA expression plasmid was in a pcDNA.3 backbone40 (link) (Invitrogen), and green fluorescent protein (GFP) expression plasmid was from Clontech.
Dombernowsky S.L., Samsøe-Petersen J., Petersen C.H., Instrell R., Hedegaard A.M., Thomas L., Atkins K.M., Auclair S., Albrechtsen R., Mygind K.J., Fröhlich C., Howell M., Parker P., Thomas G, & Kveiborg M. (2015). The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17. Nature communications, 6, 7518.
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