Ir spirit fourier transform infrared ft ir spectrometer

Materials and Equipment. Reagents were purchased from Chemline Scientific Inc. and used as received. The Coffea arabica L. husk samples were obtained from the Agronomy Department in UPLB. The samples were authenticated in the UPLB Museum of Natural History, College of Forestry and Natural Resources, UP Los Baños. The husks were sun-dried to remove the moisture before being cut into smaller pieces. The lotion and sunscreen were commercially available products obtained from local stores. For the UV-Visible (UV-Vis) Spectrometry analysis, Thermo Scientific™ Multiskan™ GO Microplate Spectrophotometer from Thermo Fisher Scientific was used. For ATR-FTIR analysis, IRSpirit Fourier transform infrared (FTIR) spectrometer from Shimadzu was used. The lotion that was used in this study is NIVEA Moisturizing Cream and the sunscreen lotion was Nivea Sun Protect and Moisture SPF 30.
Lignin Preparation. Lignin was isolated from Coffea arabica L. based on the method of Björkman (Björkman, 1954) (link). In this procedure, the dried coffee husk was washed with ethanol to remove impurities. It was then extracted twice with 96:4 dioxane:water ratio by volume for 48 hours with constant stirring. The extract was then dried using rotary evaporator (40°C) to afford the MWL.