Autoflex 2 maldi tof ms

Manufactured by Bruker

Sourced in Germany

The AutoFlex II MALDI-TOF MS is a mass spectrometry instrument manufactured by Bruker. It utilizes matrix-assisted laser desorption/ionization (MALDI) technology and time-of-flight (TOF) mass analysis to provide accurate mass measurements of various analytes, including proteins, peptides, and other biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Initiator alstra peptide synthesizer, by Biotage (1 mentions) Flexcontrol software, by Bruker (1 mentions) Flexanalysis, by Bruker (1 mentions)

Spelling variants of this product

MALDI-TOF MS (808 citations) MALDI-TOF (318 citations) Autoflex II (56 citations) Autoflex MALDI-TOF MS (28 citations) Autoflex MALDI-TOF (24 citations) Autoflex II TOF/TOF (8 citations) Autoflex II MALDI-TOF (3 citations) Autoflex II MALDI TOF/TOF (2 citations)

2 protocols using autoflex 2 maldi tof ms

Synthesis and Characterization of Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

All peptides were synthesized by
the standard Fmoc solid-phase strategy using Initiator Alstra peptide
synthesizer (Biotage, Uppsala, Sweden). After cleavage from the resin,
the peptides were purified by reverse-phase high-performance liquid
chromatography (RP-HPLC). The purity of the peptides was above 95%
determined by RP-HPLC. The molecular mass of the peptides was measured
by AutoFlex II MALDI-TOF MS (Bruker Daltonics, MA). The abbreviations
or assigned names, the sequences, molecular masses, purity, and chromatographic
spectrums for each of the peptides are shown in the Supporting Information in Table S1 and Figure S1.

Akonnor A., Makise M, & Kuniyasu A. (2023). CXCR4-Targeted Necrosis-Inducing Peptidomimetic for Treating Breast Cancer. ACS Omega, 8(27), 24467-24476.

+ Open protocol

+ Expand

Characterization of Isolate CH-KOV3 by MALDI-TOF MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolate CH-KOV3 was characterized using a Bruker Autoflex II MALDI-TOF MS (Bremen, Germany) equipped with a UV nitrogen laser (337 nm) and a dual microchannel microplate detector. Together with whole (intact) bacteria, crude cell extracts were prepared accordingly [30] and also analyzed as previously described [31] . Spectra were recorded by Flex Control software (Bruker Daltonics, Bremen, Germany), and samples were analyzed in six replicates using Flex Analysis (Bruker Daltonics, Bremen, Germany). For each measurement, at least 3,000 individual spectra (300 shots at laser power from 10 different points of a dried sample spot) were collected and averaged to obtain MALDI-TOF MS spectrum. External calibration was performed with protein standards (Bruker Protein Test Standard, Bruker Daltonics, Bremen, Germany). MALDI BioTyper db 6903 software was used for the protein profile analysis by pattern matching with the libraries.

Djurić A., Gojgić-Cvijović G., Jakovljević D., Kekez B., Kojić J.S., Mattinen M.L., Harju I.E., Vrvić M.M, & Beškoski V.P. (2017). Brachybacterium sp. CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. International journal of biological macromolecules, 104(Pt A).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!