We used H3K27me3 and H3K27ac ChIP-seq data, and RNA-seq data, to evaluate the performance of HMCan-diff in two experiments: (i) lung adenocarcinoma (A549 cell line) compared to normal lung tissue; (ii) human breast adenocarcinoma (MCF7 cell line) compared to primary human mammary epithelial cells (HMEC cell line). The A549 data were generated using the Diagenode polyclonal antibody specific to H3K27me3 (C15410069) and the Abcam antibody specific to H3K27ac (ab4729). Data is deposited in GEO with accession number GSE75903. Chromatin preparation and ChIP were performed with the Ideal ChIP-seq kit for histones according to the supplier's protocol (Diagenode). Data for the lung tissue were obtained from the epigenomic roadmap database, and data for the MCF7 and HMEC cell lines were obtained from ENCODE.
C15410069
Manufactured by Abcam
Sourced in United States
C15410069 is a tool designed for protein quantification and purification. It functions as an immunoaffinity resin that enables the capture and isolation of target proteins from complex biological samples.
Automatically generated - may contain errors
2 protocols using c15410069
1
Evaluating HMCan-diff for Cancer Epigenomics
2
Mapping Histone Modifications and CTCF Binding
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Chromatin immunoprecipitations (ChIPs) were performed on confluent ADSCs and analyzed as described [59 (link)]. ChIP grade Diagenode (Denville, NJ, USA) rabbit anti-H3K4me3 (C15410003), rabbit anti-H3K4me2 (pAb-035-050), rabbit anti-H3K27me3 (C15410069), and Abcam (Waltham, MA, USA) rabbit anti-H3K27Ac (ab4729) were used to study the histone marks. The CTCF antibody from Active Motif (Carlsbad, CA, USA) (61311) was used to study CTCF boundary sites. Rabbit anti-RNAPII (abcam, ab5095) was used to study the binding of the elongating form of RNA polymerase II (Serine 2 phospho form).
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