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Scanasyst fluid afm probes

Manufactured by Bruker
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The ScanAsyst-Fluid+ AFM probes are a type of atomic force microscopy (AFM) probe designed for imaging in fluid environments. They are manufactured by Bruker for use with their AFM instruments. The probes are optimized for high-resolution imaging of samples in liquid solutions.

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Lab products found in correlation

Multimode 8 system, by Bruker (1 mentions) Nanoscope 5 controller, by Bruker (1 mentions) Te2000 u microscope, by Nikon (1 mentions)

Close spelling variants of this product

ScanAsyst Fluid probes ScanAsyst-Fluid ScanAsyst AFM ScanAsyst probe AFM probes ScanAsyst

2 protocols using scanasyst fluid afm probes

1

AFM Imaging of DNA Nanostructures

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AFM images were captured on a Bruker Multimode 8 system with Nanoscope V controller in a ScanAsyst in Fluid mode with ScanAsyst-Fluid+ AFM probes (Bruker, k ~ 0.7 N/m, tip radius <10 nm). Two microliters of sample was deposited on freshly cleaved mica followed by the addition of 48 μL of 1x TAE with 12.5 mM Mg2+ for 2 min. One mM NiCl2 buffer can be used to enhance the adsorption of DNA nanostructures on the mica surface.
Smolková B., MacCulloch T., Rockwood T.F., Liu M., Henry S.J., Frtús A., Uzhytchak M., Lunova M., Hof M., Jurkiewicz P., Dejneka A., Stephanopoulos N, & Lunov O. (2021). Protein Corona Inhibits Endosomal Escape of Functionalized DNA Nanostructures in Living Cells. ACS applied materials & interfaces, 13(39), 46375-46390.
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2

Atomic Force Microscopy in Air and Water

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Measurements were performed using an Icon and Bioscope Catalyst AFMs (both by Bruker-Nano Inc) equipped with NanoScope V controller. Bioscope catalyst was mounted on the top of Nikon TE2000U microscope. Standard Bruker ScanAsyst-Air AFM cantilevers were used to obtain all images shown in this work. The images shown in this work were acquired while scanning in air using standard air probe holders. Exactly the same procedure is applicable for scanning in water. The only difference will by using of Bruker ScanAsyst-Fluid AFM probes. To implement ringing mode, one needs to collect unfiltered (raw) signals from AFM. Details of such implementation are described in Supplementary note 1.
Dokukin M.E, & Sokolov I. (2017). Nanoscale compositional mapping of cells, tissues, and polymers with ringing mode of atomic force microscopy. Scientific Reports, 7, 11828.
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