E0771 cells were stained with PE-conjugated anti-mouse CD284 (Biolegend, 117605) and Alexa Fluor 488-conjugated anti-mouse CD282 (Biolegend, 121807). To analyze CD8+ T cell infiltration in tumor tissues, E0771 tumors from WT and Bgn KO mice were dissected and digested with collagenase II and DNase I. Single-cell suspensions were stained with FITC-conjugated anti-CD45 antibody (Biolegend, 103115) and APC-conjugated anti-CD8a antibody (Biolegend, 100711). CD45 + CD8+ T cells were analyzed using a FACS Aria II. Data was analyzed using FlowJo V10 software (Tree Star Inc.).
Alexa fluor 488 conjugated anti mouse cd282
Manufactured by BioLegend
Alexa Fluor 488-conjugated anti-mouse CD282 is a fluorescent-labeled antibody that binds to the CD282 (Toll-like receptor 2) protein expressed on the surface of mouse cells. This product is designed for use in flow cytometry and other immunoassay applications.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 conjugated anti mouse cd282
1
Quantifying CD8+ T Cell Infiltration in Tumor Tissue
2
Immune Cell Profiling in Tumor Samples
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E0771 cells were stained with PE-conjugated anti-mouse CD284 (Biolegend, 117605) and Alexa Fluor 488-conjugated anti-mouse CD282 (Biolegend, 121807). To analyze CD8 + T cell in ltration in tumor tissues, E0771 tumors from WT and Bgn KO mice were dissected and digested with collagenase II and DNase I. Single-cell suspensions were stained with FITC-conjugated anti-CD45 antibody (Biolegend, 103115) and APC-conjugated anti-CD8a antibody (Biolegend, 100711). CD45 + CD8 + T cells were analyzed using a FACS Aria II. Data was analyzed using FlowJo V10 software (Tree Star Inc.).
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