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Percp conjugated anti cd3 antibody

Manufactured by BD
Sourced in United States

The PerCP-conjugated anti-CD3 antibody is a laboratory reagent used for the identification and enumeration of T cells in flow cytometry analysis. It binds specifically to the CD3 receptor, a protein complex found on the surface of T cells, and is conjugated to the PerCP fluorescent dye to enable detection and quantification of T cell populations.

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2 protocols using percp conjugated anti cd3 antibody

1

Peripheral Blood and Decidua Lymphocyte Immunophenotyping

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One hundred microliters of peripheral blood and decidua lymphocytes (1X106 cells/100 μl) was labeled with 20 μl of FITC-conjugated anti-CD56 monoclonal antibody (Clone B159, Becton-Dickinson, Franklin Lakes, NJ, USA), PE-conjugated anti-CD314 (NKG2D) (Clone1D11, eBioscience, San Diego, CA, USA), anti-CD335 (NKp46) (Clone BAB281, BECKMAN COULTER, Brea, CA, USA), anti-CD336 (NKp44) (Clone Z231, BECKMAN), anti-CD337 (NKp30) (Clone Z25, BECKMAN), PerCP-conjugated anti-CD3 antibody (Clone SK7, Becton-Dickinson), and PerCP-conjugated anti-CD16 (Clone 3G8, BioLegend, San Diego, CA, USA).
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2

Double Staining of PBL Cells

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For double staining, PBL were incubated simultaneously with 2 μM DCFH-DA and PerCP-conjugated anti-CD3 antibody (Becton Dickinson) for 20 minutes and analyzed on a FACscan flow cytometer.
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