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Topotecan

Manufactured by Enzo Life Sciences
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Topotecan is a topoisomerase I inhibitor, a class of compounds that interfere with the action of the enzyme topoisomerase I, which is involved in the relaxation of supercoiled DNA. Topotecan is commonly used as a research tool to study the role of topoisomerase I in various cellular processes.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Menadione, by Merck Group (1 mentions) Cm dcfda, by Thermo Fisher Scientific (1 mentions) Mitopy1, by Bio-Techne (1 mentions) Mitosox, by Thermo Fisher Scientific (1 mentions) Antimycin a, by Merck Group (1 mentions) N ethylmaleimide, by Merck Group (1 mentions) Rotenone, by Merck Group (1 mentions) Mito tempo, by Enzo Life Sciences (1 mentions)

2 protocols using topotecan

1

Cell Culture and RNAi Transfection

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K562 lymphoblasts (ATCC CCL-243) were obtained from ATCC and cultured in RPMI medium with 10% fetal calf serum. GM639 cells (gift of Dr. Ray Monnat, University of Washington), were cultured in DMEM medium with 10% fetal calf serum. 5-aza-dC was obtained from Calbiochem/EMD Millipore; the PARP inhibitor BMN-673 (talazoparib), from ApexBio; and the topoisomerase 1 inhibitor topotecan from Enzo Life Sciences. Ambion Silencer® Select Validated siRNA to TP53 (#4427038, siRNA ID: s605) and Negative Control No. 2 siRNA (siNT2, #4390846) were purchased from Thermo Fisher. siRNA transfection of GM639 cells was performed using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s protocol.
Kiianitsa K., Zhang Y, & Maizels N. (2020). Treatment of human cells with 5-aza-dC induces formation of PARP1-DNA covalent adducts at genomic regions targeted by DNMT1. DNA repair, 96, 102977.
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2

Mitochondrial Oxidative Stress Assays

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Menadione, N-ethylmaleimide (NEM), rotenone, and antimycin A were from Sigma-Aldrich. Topotecan and Mito-TEMPO were from Enzo Life Sciences. MitoSOX and CM-DCFDA were from Invitrogen. MitoPY1 was from Tocris (4428).
Zhang Y., Lee J.H., Paull T.T., Gehrke S., D’alessandro A., Dou Q., Gladyshev V.N., Schroeder E.A., Steyl S.K., Christian B.E, & Shadel G.S. (2018). Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity. Science signaling, 11(538), eaaq0702.
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