Incucyte scratch wound assay system for 96 well plates

Manufactured by Sartorius

The IncuCyte® Scratch Wound Assay system for 96 well plates is a tool used to conduct in-vitro cell migration and wound healing experiments. The system provides automated, real-time, and quantitative analysis of cell movement and wound closure over time within a 96-well microplate format.

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Lab products found in correlation

96 well image lock plate, by Sartorius (2 mentions) 96 pin incucyte woundmaker tool, by Sartorius (2 mentions)

2 protocols using incucyte scratch wound assay system for 96 well plates

Cell Migration Assay with IncuCyte

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Cell migration was determined using the IncuCyte® Scratch Wound Assay system for 96 well plates (Essen Bioscience). 96 well image lock plates (Essen Bioscience) were PLL coated prior to seeding of 40,000 cells per well. Wound application was performed 24 h post seeding using the 96-pin IncuCyte WoundMaker tool (Essen Bioscience) and indicated treatments were applied simultaneously. Measurement was performed in technical replicates (n = 4–8) and repeated as independent, biological experiments as stated for the respective experiment in figure legends.

Kiweler N., Delbrouck C., Pozdeev V.I., Neises L., Soriano-Baguet L., Eiden K., Xian F., Benzarti M., Haase L., Koncina E., Schmoetten M., Jaeger C., Noman M.Z., Vazquez A., Janji B., Dittmar G., Brenner D., Letellier E, & Meiser J. (2022). Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis. Nature Communications, 13, 2699.

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Cell Migration Assay Protocol

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Cell migration was determined using the IncuCyte® Scratch Wound Assay system for 96 well plates (Essen Bioscience). 96 well image lock plates (Essen Bioscience) were PLL coated prior to seeding of 40,000 cells per well. Wound application was performed 24 h post seeding using the 96-pin IncuCyte WoundMaker Tool (Essen Bioscience) and indicated individual treatment conditions were applied simultaneously. Measurement for individual treatment conditions was performed in biological replicates (n = 4 -8) and repeated as independent experiments as stated for the respective experiment (see figure legends).

Kiweler N., Delbrouck C., Neises L., Pozdeev V.I., Soriano‐Baguet L., Feng X., Benzarti M., Haase L., Schmoetten M., Jäger C., Noman M.Z., Vázquez A., Janji B., Dittmar G., Brenner D., Letellier E., & Meiser J. (2021). Mitochondrial One-Carbon Flux has a Growth-Independent Role in Promoting Breast Cancer Metastasis.

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