NETs were quantified using the Quanti-iT™ PicoGreen® dsDNA kit (Invitrogen, UK) and using a capture ELISA. Streptavidin-coated plates (Fisher Scientific, UK) were coated with an anti-MPO capture antibody (Abcam, UK) overnight at 4° C and blocked with 0.5% bovine serum albumin for 1 h at 37° C. Neutrophil supernatants were incubated for 2 h at 37° C. Further 1 h incubations were performed with an anti-citrullinated histone H3 detection antibody (Abcam, UK) and HRP-conjugated secondary antibody (Dako, UK). SureBlue TMB Microwell Peroxidase Substrate (KPL, UK) was then added and incubated in the dark at 37° C for 20 min and then stopped by the addition of TMB stop solution (KPL, UK). Absorbance was read at 450 nm using a Tecan GENios Spectra FLUOR plate reader (Tecan UK Ltd., UK).
Anti citrullinated histone h3 detection antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-citrullinated histone H3 detection antibody is a laboratory reagent designed to detect the presence of citrullinated histone H3 in samples. This antibody can be used to identify and quantify citrullinated histone H3, which is a post-translational modification associated with various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti mpo capture antibody, by Abcam (2 mentions)
Genios spectra fluor plate reader, by Tecan (2 mentions)
Quanti it picogreen dsdna kit, by Thermo Fisher Scientific (2 mentions)
Streptavidin coated plate, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated secondary antibody, by Agilent Technologies (2 mentions)
2 protocols using anti citrullinated histone h3 detection antibody
1
Quantification of Neutrophil Extracellular Traps
2
Quantification of Neutrophil Extracellular Traps
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NETs was quantified using the Quanti-iT™ PicoGreen® dsDNA kit (Invitrogen, UK). Supernatants were also tested using a capture ELISA. Streptavidin-coated plates (Fisher Scientific, UK) were coated with an anti-MPO capture antibody (Abcam, UK) overnight at 4 o C and blocked with 0.5% bovine serum albumin for 1 hour at 37 o C. Neutrophil supernatants were incubated for 2 hours at 37 o C. Further 1 hour incubations were performed with an anticitrullinated histone H3 detection antibody (Abcam, UK) and HRP-conjugated secondary antibody (Dako, UK). SureBlue TMB Microwell Peroxidase Substrate (KPL, UK) was then added and incubated in the dark at 37 o C for 20 minutes and then stopped by the addition of TMB stop solution (KPL, UK). Absorbance was read at 450nm using a Tecan GENios Spectra FLUOR plate reader (Tecan UK Ltd., UK).
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