Pe conjugated cd150

Manufactured by Thermo Fisher Scientific

PE-conjugated CD150 is a fluorescently labeled antibody that binds to the CD150 (SLAM) cell surface antigen. CD150 is a co-stimulatory molecule expressed on the surface of various immune cell types, including T cells, B cells, and dendritic cells. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD150-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Apc cy7 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Pe cy7 conjugated sca 1, by Thermo Fisher Scientific (1 mentions) Collagenase type 1, by Worthington (1 mentions) Collagenase type 4, by Worthington (1 mentions) Lsrfortessa, by BD (1 mentions) Abc total antibody compensation bead kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD150 (5 citations) CD150-PE (2 citations)

2 protocols using pe conjugated cd150

Isolation and Identification of Fetal Mouse LT-HSCs

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Fetal livers were isolated from mouse embryos at E14.5 under a dissecting microscope, and cell suspensions were prepared in PBS containing 2% FBS by repeatedly flushing through needles ranging from 18‐ to 27‐gauge. The cells were then passed through a nylon mesh with a pore size of 70 µm, and red blood cells were lysed using RBC lysis buffer (00‐4333‐57, eBioscience).
For staining LT‐HSCs, the cells were first incubated with Fc Block followed by biotin‐conjugated lineage marker antibodies (CD3e, CD4, CD5, CD8a, B220, Gr‐1, Ter119, and CD11b), followed by APC‐Cy7‐conjugated streptavidin, PE‐Cy7‐conjugated Sca‐1, APC‐conjugated CD11b, PE‐conjugated CD150, and Percp‐Cy5.5‐conjugated CD48 antibodies.^[⁹^] All antibodies were purchased from eBioscience or BioLegend. FACS data were analyzed using FlowJo software.

He X., Ge C., Xia J., Xia Z., Zhao L., Huang S., Wang R., Pan J., Cheng T., Xu P., Wang F, & Min J. (2023). The Zinc Transporter SLC39A10 Plays an Essential Role in Embryonic Hematopoiesis. Advanced Science, 10(17), 2205345.

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Murine Bone Marrow Cell Digestion

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Lin-murine bone marrow cells were resuspended in 100 µL degradation buffer (50 mM HEPES, 150 mM NaCl, 10 mM CaCl2, pH = 7.4) and exposed to either 100 Units Collagenase Type I, 100 Units of Collagenase Type IV (Worthington Biochemical, Lakewood, NJ), 10 µM SortaseA (#13101, ActiveMotif, Carlsbad, CA) with 15 mM GGG, and a control with buffer only. After 1 h at 37 °C, the degradation reaction was quenched with PBS + 5% FBS and centrifuged at 300 rcf x 10 min. The pellet was resuspended in PBS + 5% FBS for staining. After Fc blocking, cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL) was used for dead cell exclusion. All samples were run for a minimum of 25,000 live events. Compensation was setup using AbC™ Total Antibody Compensation Bead Kit (#A10513, ThermoFisher). All antibodies were supplied by eBioscience (San Diego, CA) and are as follows: PE-conjugated CD150

Gilchrist A.E., Serrano J.F., Ngo M.T., Hrnjak Z., Kim S., & Harley B.A. (2021). Encapsulation and recovery of murine hematopoietic stem and progenitor cells in a thiol-crosslinked maleimide-functionalized gelatin hydrogel.

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