Precellys24 beat beading system

Manufactured by Bertin Technologies

Sourced in France

The Precellys24 is a beat-beading system used for the efficient disruption and homogenization of biological samples, such as tissues, cells, and microorganisms. The system utilizes high-speed reciprocal motion to agitate samples with metal beads, effectively breaking down the samples for subsequent analysis.

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Lab products found in correlation

Mouse cytokine array panel a kit, by R&D Systems (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

2 protocols using precellys24 beat beading system

Profiling Cytokine and Chemokine Responses

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Cremaster muscles were homogenized in PBS containing 0.1% Triton and 1% Halt Protease and Phosphatase Inhibitor cocktail and mechanically dissociated using the Precellys24 beat-beading system (Bertin Technologies, France). The cytokine and chemokine expression profiles of these samples (pooled from 3 mice/group) were analyzed using a Mouse Cytokine Array Panel A kit (R&D Systems, Abingdon, Oxford) as per manufacturers’ instructions. The CXCL1 content of these tissues was further validated by ELISA (R&D Systems; sensitivity: 2 pg/mL). Chemokine content was expressed as per unit weight of tissue.

Barkaway A., Rolas L., Joulia R., Bodkin J., Lenn T., Owen-Woods C., Reglero-Real N., Stein M., Vázquez-Martínez L., Girbl T., Poston R.N., Golding M., Saleeb R.S., Thiriot A., von Andrian U.H., Duchene J., Voisin M.B., Bishop C.L., Voehringer D., Roers A., Rot A., Lämmermann T, & Nourshargh S. (2021). Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity, 54(7), 1494-1510.e7.

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Quantification of IL-17A and CXCL1 in Mouse Cremaster

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Anaesthetised (isofluorane 3%) mice were injected i.s. with TNF (300 ng, R&D Systems) in 400 µl PBS. Control animals received PBS only. Cremaster muscles were harvested 4 h later. Tissues were homogenised in PBS containing 0.1% Triton X-100 and 1% Halt Protease and Phosphatase Inhibitor cocktail (ThermoFisher) and mechanically dissociated using the Precellys24 beat-beading system (Bertin Technologies). Levels of IL-17A & CXCL1 were analysed as per the manufacturer’s instructions by ELISA (R&D Systems, sensitivity: 16 and 2 pg/ml respectively). The quantity of mediators detected in tissues was normalised to protein content as determined using a BCA assay (Thermo Fisher).

Joulia R., Guerrero-Fonseca I.M., Girbl T., Coates J.A., Stein M., Vázquez-Martínez L., Lynam E., Whiteford J., Schnoor M., Voehringer D., Roers A., Nourshargh S, & Voisin M.B. (2022). Neutrophil breaching of the blood vessel pericyte layer during diapedesis requires mast cell-derived IL-17A. Nature Communications, 13, 7029.

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