Argon 99.995

Manufactured by Linde

Sourced in Spain

Argon 99.995% is a high-purity gas supplied by Linde. It is a colorless, odorless, and inert gas that is commonly used in various laboratory applications. This product meets the specification of 99.995% purity, ensuring a consistent and reliable gas supply for your laboratory needs.

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Lab products found in correlation

5 protocols using argon 99.995

UHPLC-MS/MS Protocol for Analyte Characterization

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Characterization and determination of analytes were performed on an Acquity UHPLC system from Waters (Milford, MA, USA) interfaced to a Xevo TQS triple quadrupole mass spectrometer from Waters (Manchester, UK) equipped with a T-Wave and electrospray ionization interface operated in positive mode (ESI+). Chromatographic separation was achieved using an Acquity UPLC BEH C18 column (1.7 μm, 50 × 2.1 mm), also from Waters, at a flow rate of 0.3 mL min⁻¹ and 40 °C. Sample manager was kept at 5 °C. Mobile phase consisted of 5 mM ammonium acetate and 0.01% formic acid in H₂O (phase A) and MeOH (phase B). The gradient of phase B applied was as follows: initial conditions at 10%, linear increase to 90% in 3 min, 90% (3–3.5 min), 10% (3.6–6 min) for equilibration of column.
Cone and desolvation gas (dry nitrogen) flows were set to 250 and 1200 L h⁻¹ respectively. For operation in MS/MS mode, argon 99.995% (Praxair, Madrid, Spain) was used as collision gas, kept at 4·10⁻³ mbar and 0.15 mL/min in the collision cell. Capillary voltage was 3.0 kV, source and desolvation temperatures were 150 °C and 650 °C, respectively, and dwell times of 0.01 s/transition were selected.
All data were acquired using MassLynx v4.1 software (Waters, Manchester, UK) and processed using Masslynx and Microsoft’s Excel spreadsheet software.

Pitarch-Motellón J., Bijlsma L., Sancho Llopis J.V, & Roig-Navarro A.F. (2021). Isotope pattern deconvolution as a successful alternative to calibration curve for application in wastewater-based epidemiology. Analytical and Bioanalytical Chemistry, 413(13), 3433-3442.

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UHPLC-MS/MS Quantification of Compounds

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A Waters Acquity UHPLC system (Milford, MA, USA) was interfaced to a triple quadrupole mass spectrometer (Xevo TQS, Waters Micromass, Manchester, UK) equipped with Z-Wave devices and an electrospray ionization interface (ESI) operated in positive-ion mode. The chromatographic separation was performed using an Acquity UPLC BEH C 18 column, 1.7 mm, 50 mm Â 2.1 mm (i.d.)
(Waters) at a flow rate of 0.3 mL min À1 . The mobile phases used were water with 5 mM ammonium acetate and 0.01% formic acid (solvent A) and MeOH with 0.01% formic acid (solvent B). The percentage of B changed linearly as follows: 0 min, 10%; 3 min, 90%; 3.5 min, 90%; 3.6 min, 10%; 6 min 10%, equilibration of the column. Cone gas as well as desolvation gas was dry nitrogen, with flows set to 150 and 800 L h À1 , respectively. For operation in MS/MS mode, collision gas was argon 99.995% (Praxair, Madrid, Spain) with a pressure of 4 Â 10À3 mbar in the collision cell (0.15 mL min À1 ). Further parameters optimised were: capillary voltage, 3.0 kV; source temperature, 150 C and desolvation temperature, 650 C. Dwell times of 0.01 s/transition were automatically selected.
All acquired data were processed using MassLynx v4.1 software (Waters, Manchester, UK).

Bade R., Bijlsma L., Sancho J.V., Baz-Lomba J.A., Castiglioni S., Castrignanò E., Causanilles A., Gracia-Lor E., Kasprzyk-Hordern B., Kinyua J., McCall A.K., van Nuijs A.L.N., Ort C., Plósz B.G., Ramin P., Rousis N.I., Ryu Y., Thomas K.V., de Voogt P., Zuccato E, & Hernández F. (2017). Liquid chromatography-tandem mass spectrometry determination of synthetic cathinones and phenethylamines in influent wastewater of eight European cities. Chemosphere, 168.

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UHPLC-MS/MS for Metabolite Quantification

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UHPLC-MS/MS sample analysis was performed using a Waters Acquity H-class UPLC system (Waters Corporation, MA, USA) coupled to a triple quadrupole mass spectrometer (Xevo TQS, Waters Micromass, Manchester, UK) equipped with an electrospray ionization source (ESI) operated in positive ionization mode. Chromatographic separation was performed by means of an Acquity UPLC BEH C 18 column (1.7 m, 50 × 2.1 mm) from Waters at a flow rate of 300 l min -1 . Column temperature was kept at 40 • C and sample manager was kept at 7 • C. Elution was performed with gradient of A: H 2 O 0.01% HCOOH and B: MeOH 0.01% HCOOH, as follows: 0 min 10% B, 2 min 60% B, 2.50 min 90% B, 3.50 min 90% B, 3.60 min 10% B until 5.50 min for re-equilibrating the column for the next injection. Cone and desolvation gas were dry nitrogen set to 250 L h -1 and 1200 L h -1 , respectively. For the operation of MS/MS mode, collision gas was argon 99.995% (Praxair, Madrid, Spain) set to 0.15 mL min -1 . Source temperature was kept at 120 • C and capillary voltage was established at 3.0 kV. All data was acquired and processed using MassLynx v4.1 software (Waters, Manchester, UK).

Celma A., Sancho J.V., Salgueiro-González N., Castiglioni S., Zuccato E., Hernández F, & Bijlsma L. (2019). Simultaneous determination of new psychoactive substances and illicit drugs in sewage: Potential of micro-liquid chromatography tandem mass spectrometry in wastewater-based epidemiology. Journal of chromatography. A, 1602.

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UPLC-QTOF MS Metabolite Profiling

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A Waters Acquity UPLC system (Waters, Milford, MA, USA) was interfaced to a hybrid quadrupole-orthogonal acceleration-TOF mass spectrometer (XEVO G2 QTOF, Waters Micromass, Manchester, UK), using a ESI (Z-Spray) interface operating in positive ion mode. The chromatographic separation was performed using an Acquity UPLC BEH C18 100 × 2.1 mm, 1.7 µm particle size column (Waters) at a flow rate of 300 µl/min. The mobile phases used were A = H 2 O and B = MeOH, both with 0.01% formic acid. The initial percentage of B was 10%, which was linearly increased to 90% in 14 min, followed by a 2 min isocratic period and, then, returned to initial conditions during 2 min. The total run time was 18 minutes. Nitrogen was used as drying gas and nebulizing gas. TOF-MS resolution was approximately 20.000 at full width half maximum at m/z 556. MS data were acquired over an m/z range of 50-1000,at 0.4 s scan time . A capillary voltage of 0.7 kV and cone voltage of 20 V were used. Collision gas was argon 99.995% (Praxair, Valencia, Spain). The desolvation temperature was set to 600 °C, and the source temperature to 135 °C. The column temperature was set to 40 °C. MS data was acquired in MS E mode, selecting a collision energy of 4eV for low energy (LE) and a ramp of 15-40eV for high energy (HE) [5, (link)18] (link).

Bade R., Bijlsma L., Sancho J.V, & Hernández F. (2015). Critical evaluation of a simple retention time predictor based on LogKow as a complementary tool in the identification of emerging contaminants in water. Talanta, 139.

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UPLC-MS/MS Peptide Quantification Protocol

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Sample analysis was performed using a Waters Acquity M-class UPLC system (Waters Corporation, MA, USA), equipped with a Peptide BEH C 18 130 Å 1.7 m 150 m × 50 mm ionKey LC separation device (Waters Corporation) and interfaced to a triple quadrupole mass spectrometer (Xevo TQS, Waters Micromass, Manchester, UK) operated in positive ionization mode. iKey device was kept at 40 • C and sample manager was kept at 7 • C. Chromatographic separation was performed with gradient of A: H 2 O 0.01% HCOOH and B: MeOH 0.01% HCOOH, as follows: 0 min 30% B, 2 min 60% B, 2.10 min 90% B, 3.10 min 90% B, 3.20 min 30% B until 5.50 min for re-equilibrating the column for next injection. Flow rate was established at 3 L min -1 . Cone and nebulizer gas were dry nitrogen set to 250 L h -1 and 7 bar, respectively. For the operation of MS/MS mode, collision gas was argon 99.995% (Praxair, Madrid, Spain) set to 0.15 mL min -1 . Source temperature was kept at 120 • C and capillary voltage was established at 3.5 kV. All data was acquired and processed using MassLynx v4.1 software (Waters, Manchester, UK).

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