Whey produced during Swiss cheese production was obtained from the Key Laboratory of Dairy Science, Northeast Agricultural University, Harbin, China, and all whey samples were stored at -20°C until processing. Sweet whey was made using liquid bovine rennet (containing 95% chymosin and 5% pepsin) obtained from New England Cheesemaking Supply Company, as earlier described by Timón et al. (2014) (link). Industrial alcohol active dry yeast S. cerevisiae (containing rhizopus, α-amylase, glucoamylase, phytase) was purchased from Angel Yeast Co. Ltd. Lactase (10,000 acid lactase unit/g, 37°C, pH 6) was bought from the Nanning Pangbo Bioengineering Co. Ltd. We purchased α-dlactose monohydrate, d-galactose, and d-(+)-glucose compounds from Shanghai Gold Wheat Biological Technology Company, prepared from commercially available standards, purity grade ≥98%. Ethyl acetate, acetaldehyde, acetic acid, methanol, n-butanol, 1-propanol, 2-butanol, amyl alcohol, isobutyl alcohol, isoamyl alcohol, and 4-nanonol (GC standard purity) were purchased from Sigma-Aldrich as internal standards.
Isobutyl alcohol
Manufactured by Merck Group
Sourced in Germany
Isobutyl alcohol is a colorless, flammable liquid organic compound with the chemical formula C4H10O. It is a primary alcohol that is used as a solvent and chemical intermediate in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
1 propanol, by Merck Group (2 mentions)
Isoamyl alcohol, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Acetaldehyde, by Merck Group (1 mentions)
N butanol, by Merck Group (1 mentions)
Amyl alcohol, by Merck Group (1 mentions)
2 butanol, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
7683b autosampler, by Agilent Technologies (1 mentions)
2 protocols using isobutyl alcohol
1
Whey Bioconversion by Yeast and Enzymes
2
Analysis of Alcoholic Fermentation By-products
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Selected by-products of alcoholic fermentation (acetaldehyde, ethyl acetate, methanol, 1-propanol, isobutyl alcohol, and isoamyl alcohol were purchased from Sigma-Aldrich, Steinheim, Germany) in the distillate were analyzed on an Agilent Technologies 7890A GC with a flame ionization detector (FID) and a capillary column with DB-FFAP phase, dimensions 30 m × 0.53 mm × 1 µm, working with an Agilent Technologies 7683B autosampler, with a 10 µL syringe. A validated method, in accordance with PN-EN ISO/IEC 17025 guidelines [45] , was applied for the analysis. The contents of the compounds were calculated on the basis of calibration curves. The results obtained from the chromatographic analyses expressed in mg/100 mL of the sample were converted into g/L alc. 100% vol. according to the formula:
where: W x -concentration of the analyte, (g/L alc. 100 % vol.); C x -concentration of the given analyte in the test sample obtained from the chromatographic analysis report, (mg/100 mL); M-alcoholic strength, (% vol.). The arithmetic mean of three parallel determinations carried out on the same sample was taken as the final result.
where: W x -concentration of the analyte, (g/L alc. 100 % vol.); C x -concentration of the given analyte in the test sample obtained from the chromatographic analysis report, (mg/100 mL); M-alcoholic strength, (% vol.). The arithmetic mean of three parallel determinations carried out on the same sample was taken as the final result.
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