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Alamar blue 10x ready to use solution

Manufactured by Merck Group
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Sourced in Ireland

Alamar blue (AB) (10X ready to use solution) is a lab equipment product manufactured by Merck Group. It is a resazurin-based solution that can be used to assess cell viability and proliferation. The 10X concentrated solution can be diluted for use in various cell-based assays.

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Lab products found in correlation

3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) A549 human lung adenocarcinoma cells, by American Type Culture Collection (2 mentions) Doxorubicin hydrochloride powder, by Merck Group (2 mentions)

2 protocols using alamar blue 10x ready to use solution

1

Cytotoxicity Assay in A549 Cells

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A549 human lung adenocarcinoma cells with the alveolar type II phenotype were obtained from ATCC (Manassas, VA, USA). Doxorubicin hydrochloride ® powder (Sigma Life Sciences, Ireland) was diluted in1mL sterile water to the required concentration (17.25 mM).
Alamar blue (AB) (10X ready to use solution) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma Aldrich, Ireland.
For cytotoxicity assays, an AB/MTT solution, 1.5mL of AB and 3 mL of MTT stock solution (2.5mg/mL, 25mg MTT/10mL PBS) in 30mL of fresh medium was prepared prior to the tests.
Farhane Z., Bonnier F., Howe O., Casey A, & Byrne H.J. (2018). Doxorubicin kinetics and effects on lung cancer cell lines using in vitro Raman micro-spectroscopy: binding signatures, drug resistance and DNA repair. Journal of biophotonics, 11(1).
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2

Cytotoxicity Assay in A549 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 human lung adenocarcinoma cells with the alveolar type II phenotype were obtained from ATCC (Manassas, VA, USA). Doxorubicin hydrochloride ® powder (Sigma Life Sciences, Ireland) was diluted in1mL sterile water to the required concentration (17.25 mM).
Alamar blue (AB) (10X ready to use solution) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma Aldrich, Ireland.
For cytotoxicity assays, an AB/MTT solution, 1.5mL of AB and 3 mL of MTT stock solution (2.5mg/mL, 25mg MTT/10mL PBS) in 30mL of fresh medium was prepared prior to the tests.
Farhane Z., Bonnier F, & Byrne H.J. (2017). Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines. Analytical and bioanalytical chemistry, 409(5).
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