Blood samples were drawn for assays after overnight fasting. Serum levels of total and HDL cholesterol and triglycerides were enzymatically determined (Qualigent, Sekisui Medical, Tokyo, Japan) using automated instrumentation based on previously described assays12 (link)). Hypertension was defined as systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg or treatment with antihypertensive medications. The presence of diabetes was defined as described previously by the Japan Diabetes Society13 ). Smoking status was defined as current smoking habits. Left ventricular ejection fraction (LVEF) was calculated either by modified Simpson's method or by Teichholz method. We defined major adverse cardiac events (MACE) as either all-cause death, including cardiac death, ST elevated myocardial infarction (STEMI), non-ST elevated myocardial infarction (NSTEMI), unstable angina pectoris (UAP), staged percutaneous coronary intervention (PCI) or coronary artery bypass grafting (CABG), ischemic as well as hemorrhagic stroke, heart failure requiring hospital admission.
Qualigent
Manufactured by Sekisui
Sourced in Japan
Qualigent is a laboratory equipment product designed for precision analysis. It provides accurate and reliable data collection and measurement capabilities for various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using qualigent
1
Metabolic Biomarkers and Cardiac Outcomes
2
Serum Lipid Profiling Protocol
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Blood samples were collected after overnight fasting, and serum total cholesterol, triglycerides and high-density lipoprotein (HDL) cholesterol were assayed enzymatically with an autoanalyzer (Qualigent, Sekisui Medical, Tokyo, Japan) as previously described [11 (link)]. If triglycerides were <400 mg/dL, then LDL cholesterol concentration was calculated with the Friedewald equation; if not, then it was determined enzymatically. The initial data was obtained prior to the introduction of lipid-lowering treatments.
3
Lipid Profile Modulation by Atorvastatin and Ezetimibe
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Blood samples were collected after overnight fasting, and serum total cholesterol, triglycerides and high-density lipoprotein (HDL) cholesterol were assayed enzymatically with an autoanalyzer (Qualigent, Sekisui Medical, Tokyo, Japan). If triglycerides were < 400 mg/dL, then LDL cholesterol concentration was calculated with the Friedewald equation; if not, then it was determined enzymatically. Baseline was assessed just before the introduction of atorvastatin 10 mg/day, and lipids with atorvastatin 10 mg/day was assessed just before the introduction of ezetimibe 10 mg/day. Lipids with atorvastatin 10 mg/day and ezetimibe 10 mg/day were assessed just after the addition of ezetimibe 10 mg/day (the interval was at least 4 weeks).
4
Lipoprotein Biomarker Measurement Protocol
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LDL-C values were measured directly using QUALIGENT® (Sekisui Medical, Tokyo, Japan). RLP is estimated as the unbound fraction of plasma after incubation with immunoaffinity gel of apoB100 monoclonal antibody and apolipoprotein A-I monoclonal antibody as described12 (link)). ApoE phenotype was separated by isoelectric focusing and detected by western blot with apoE polyclonal antibody (phenotyping apoE IEF system, JOKOH, Tokyo, Japan). Apolipoprotein B-48 (apoB48) levels were determined by ELISA method using monoclonal antibody against C-terminal decapeptide of apoB4813 (link)). Serum levels of sterols, including sitosterol, lathosterol, and campesterol, were determined using gas-liquid chromatography-mass spectrometry as previously described9 (link)).
5
Serum Biomarkers Quantification Protocol
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Serum levels of triglyceride, total cholesterol, β-hydroxybutyrate, NEFA, and FGF21 were measured according to the manufacturer’s instruction. Briefly, after blood sample collection, serum samples were enzymatically processed with Qualigent (triglyceride and total cholesterol, Sekisui Medical, Tokyo, Japan), 3HB-L Test Kit (β-hydroxybutyrate, Kainos, Tokyo, Japan), and Clinimate NEFA (NEFA, Sekisui Medical, Tokyo, Japan). Then, they were measured using the Hitachi Automated Analyzer (Labospect003, Hitachi High-Technologies, Tokyo, Japan). Serum FGF21 level was measured using an FGF-21 ELISA kit (Cat #RD291108200R, BioVendor Laboratory Medicine, Brno, Czech Republic).
6
Lipid Panel Assay Protocol
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Fasting blood samples were drawn for assays either before the lipid-lowering treatment or after discontinuation of medication for at least 4 weeks. Blood samples were stored at 4 °C immediately in the pharmaceutical refrigerators (MPR-721, Panasonic healthcare, Tokyo, Japan) until the process to obtain serum and plasma for within an hour. Serum concentrations of total cholesterol (TC), TG, and HDL cholesterol (HDL-C) were determined enzymatically (Qualigent®, Sekisui Medical, Tokyo, Japan) using automated instrumentation (LABOSPECT 008, Hitachi High-Technologies, Tokyo, Japan) based on the assays previously described [11] (link), [12] (link), [13] (link). LDL-C concentrations were derived using the Friedewald formula [14] (link). Plasma were processed to ultracentrifugation immediately after the acquisition.
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