Flowlogic v8

Manufactured by Inivai Technologies

Sourced in Australia

FlowLogic-v8 is a laboratory equipment designed for flow analysis and measurement. It features advanced sensors and software for precise data collection and processing. The core function of FlowLogic-v8 is to enable researchers and scientists to accurately monitor and analyze fluid dynamics in a controlled environment.

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FlowLogic (26 citations)

5 protocols using flowlogic v8

Comprehensive Cytokine and Chemokine Profiling

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Plasmatic cytokines (IFN-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-17A, and TNF- α) and chemokine (GM-CSF) were quantified with the Human MACSPlex Cytokine 12 Kit (Miltenyi Biotec) according to the manufacturer’s instructions. Samples were acquired on FACSVerse^™cytometer (BD Biosciences) and analyzed with FlowLogic-v8 (Inivai Technologies).

Rovito R., Bono V., Augello M., Tincati C., Mainoldi F., Beaudoin-Bussières G., Tauzin A., Bianchi S., Hadla M., Yellenki V., d’Arminio Monforte A., Casola S., Borghi E., Finzi A, & Marchetti G. (2022). Association between SARS-CoV-2 RNAemia and dysregulated immune response in acutely ill hospitalized COVID-19 patients. Scientific Reports, 12, 19658.

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Analyzing Protein Synthesis in Leukemic Cells

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CD34+ cells were washed and resuspended in media with different concentrations of C646 (Sigma-Aldrich) and incubated for another 8 h after which protein synthesis was analysed with the Protein Synthesis Assay Kit Red (Abcam) according to the manufacturer’s instructions. The samples were analysed on a Beckman Coulter Cyan ADP (Beckman Coulter) and with FlowJo v10 (FlowJo LLC). The median fluorescence intensity of each condition was normalized to the DMSO control. Statistical analysis (unpaired t-test) were calculated using GraphPad Prism software (version 6).
Leukemic blast cells or SKK-1 and MOLM-13 cells were treated with different concentrations of AZA, C646, A-485, CCS1477 or DMSO and incubated for 3 or 6 h, respectively. Cells treated 1.5 h with CHX were used as a positive control. The samples were then processed using the Click-iT™ HPG Alexa Fluor™ 594 kit (Thermo Scientific) according to the manufacturer’s instructions and the protein synthesis analysed using the LSR Fortessa cytometer (BD Biosciences) and FlowLogic v8 (Inivai Technologies, Victoria, Australia).

Diesch J., Le Pannérer M.M., Winkler R., Casquero R., Muhar M., van der Garde M., Maher M., Herráez C.M., Bech-Serra J.J., Fellner M., Rathert P., Brooks N., Zamora L., Gentilella A., de la Torre C., Zuber J., Götze K.S, & Buschbeck M. (2021). Inhibition of CBP synergizes with the RNA-dependent mechanisms of Azacitidine by limiting protein synthesis. Nature Communications, 12, 6060.

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Multiparametric Flow Cytometry Analysis

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Data were acquired on a MACSQuant® Analyzer 10 or MACSQuant® X (Miltenyi Biotec) and analyzed with FlowLogic™ V.8 (Inivai Technologies) by either frequency or median fluorescence intensity (MFI). Cells were stained in CliniMACS® PBS/EDTA Buffer (Miltenyi Biotec, catalog no.: 200-070-025) with 0.5% BSA at 4°C for 10 min. Antibodies used for flow cytometry: CD137-APC (clone: REA765, Miltenyi Biotec), CD25-PE-Vio770 (clone: REA570, Miltenyi Biotec), CD69-VioBlue (clone: REA824, Miltenyi Biotec), LNGFR-PE (clone: REA844, Miltenyi Biotec), CD4-VioGreen (clone: REA623), CD8-APC-Vio770 (clone: REA734, Miltenyi Biotec), CD3-FITC (clone: REA613, Miltenyi Biotec), CD45-VioBlue (clone: REA747, Miltenyi Biotec), CD279-PE-Vio770 (clone: REA1165, Miltenyi Biotec), CD366-APC (clone: REA635, Miltenyi Biotec), CD223-FITC (clone: REA351, Miltenyi Biotec). Antibodies were used according to the manufacturer’s protocol.

Werchau N., Kotter B., Criado-Moronati E., Gosselink A., Cordes N., Lock D., Lennartz S., Kolbe C., Winter N., Teppert K., Engert F., Webster B., Mittelstaet J., Schaefer D., Mallmann P., Mallmann M.R., Ratiu D., Assenmacher M., Schaser T., von Bergwelt-Baildon M., Abramowski P, & Kaiser A.D. (2022). Combined targeting of soluble latent TGF-ß and a solid tumor-associated antigen with adapter CAR T cells. Oncoimmunology, 11(1), 2140534.

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DNA Plasmid Design and Analysis

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Design and alignment of DNA plasmids was done using SerialCloner v2.6.1 (SerialBasics) unless stated differently. Western blot membranes were analyzed using ImageStudio lite (LI-COR biosciences). Flow cytometry data was analyzed using Flowlogic v8.3 (Inivai Technologies). Microscopy images were analyzed using SoftWoRx 7.0 (Cytiva), Gen5 v3.10 (BioTek Instruments), IncuCyte GUI v2021A (Sartorius) according to instrument and subsequently handled with ImageJ. Statistical analysis and creation of graphs was done using GraphPad Prism 9 (GraphPad Spftware LLC) and Excel 2019 (Microsoft corp.). Arrangement of figures was done using CorelDraw X7 (Corel Corporation).

Bunz M., Eisele M., Hu D., Ritter M., Kammerloher J., Lampl S, & Schindler M. (2024). CD81 suppresses NF-κB signaling and is downregulated in hepatitis C virus expressing cells. Frontiers in Cellular and Infection Microbiology, 14, 1338606.

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Optimized Analysis of Protein Expression Dynamics

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Data analysis, statistical analysis, and graphing was done in RStudio v1.4.1106 for R (v4.1.2), GraphPad Prism v9.2.0 (GraphPad Software), and FlowLogic™ v8.3 (Inivai Technologies). Sigmoidal dose-response curve fits were computed on nMFIs using nonlinear regression by the variable slope (four parameters) model in GraphPad Prism v9.2.0 (GraphPad Software), using default settings (Supplementary Data 9). The significance of P_FUS1-GFP reporter expression was assessed by one-way or two-way ANOVA multivariate test and post-hoc analysis by Dunnett’s or Tukey’s multiple comparison test, respectively (Supplementary Data 9, 10). Multivariate analysis by determination of Pearson correlation coefficients for SSC-A, FSC-A and GFP MFIs and simple linear regression was done in GraphPad Prism v9.2.0 (GraphPad Software), using default settings (Supplementary Data 11). Population SSC-A histograms were overlaid and normalized to 100% using FlowLogic™ v8.3 (Inivai Technologies). To assess the significance of mating efficiencies, a two-way ANOVA multivariate test and post-hoc analysis by Dunnett’s multiple comparisons test was done (Supplementary Data 12–14). All statistical tests were done in GraphPad Prism v9.2.0 (GraphPad Software) with a default 95% confidence interval (ɑ = 0.05) applied and multiplicity-adjusted p values were reported to account for all multiple comparisons within tests.

Jensen E.D., Deichmann M., Ma X., Vilandt R.U., Schiesaro G., Rojek M.B., Lengger B., Eliasson L., Vento J.M., Durmusoglu D., Hovmand S.P., Al’Abri I., Zhang J., Crook N, & Jensen M.K. (2022). Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts. Nature Communications, 13, 6201.

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