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2 protocols using perfluorooctyl bromide pfob

1

Nanomaterials Synthesis and Characterization

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Barium sulphate (98%), eicosane (99%), octacosane (99%) were purchased from Acros Organics. Oleic acid (technical grade, 90%), calcium carbonate (99%), agar (powder, for microbiology), iron (II, III) oxide (nanopowder, 50–100 nm particle size (SEM), 97% trace metals basis, used as a magnetite XRD standard) and Crystal Violet (hexamethylpararosaniline chloride, >90%) were purchased from Sigma-Aldrich. Iron(III) chloride hexahydrate (98%) was purchased from Alfa Aesar reagents. Iron(II) sulphate heptahydrate (98%), sodium hydroxide pellets (Analytical reagent grade) and ammonium hydroxide solution (28% in water) were purchased from Fisher Scientific, and perfluorooctyl bromide (PFOB, 97%) from Fluorochem Ltd. Iohexol (Omnipaque 300) was obtained from GE healthcare. Aspirin tablets were bought commercially from Boots Ltd. Laboratory solvents were purchased from Sigma Aldrich Limited and were of analytical grade. Aqueous solutions were prepared using UHQ deionised water with a resistivity of not less than 18.2 MΩ cm−1. N2 (oxygen free) gas was purchased from BOC Ltd. and used as received. Gelatin capsules were purchased from CapsulCN, China.
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2

Nanoparticle Formulation for Drug Delivery

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Methylene chloride RPE-ACS 99.5% was provided by Carlo-Erba Reactifs (France). Sodium cholate (SC) and Nile red were purchased from Sigma-Aldrich (France). Poly (D,L-lactide-co-glycolide) -poly(ethylene glycol) (PLGA -PEG) Resomer RGP d 50105 (PLGA Mn = 45000 g/mol and 10 wt% of PEG Mn = 5000 g/mol, intrinsic viscosity 0.72 dl/g) were obtained from Boehringer-Ingelheim (Germany). Perfluorooctyl bromide (PFOB) was purchased from Fluorochem (United Kingdom). Water was purified using either a RIOS/Milli-Q system (Millipore, France) or a PURELAB Option-Q unit (ELGA LabWater).
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