The microstructure morphologies of the BNC and ENC were observed by SEM. First, the samples were fixed with 2.50% glutaraldehyde (Sigma) for 6 h at 4°C, followed by washing in PBS 3 times (5 min each) and dehydration in a graded series of ethanol (30%, 50%, 70%, 80%, 90%, and 100% ethanol for 30 min each). Later on, the samples were rinsed with deionized water 3 times (1 h each) and then lyophilized for 12 h. A thin layer of platinum alloy film was coated onto the surface of the conduits before observation. Finally, the samples were observed with a VEGA3 tungsten scanning electron microscope (TESCAN, Czech) at an accelerating voltage of 10 kV.
Vega3 tungsten scanning electron microscope
Manufactured by TESCAN
The VEGA3 is a tungsten scanning electron microscope (SEM) manufactured by TESCAN. It is designed to provide high-quality imaging and analysis of a wide range of materials and samples. The VEGA3 uses a tungsten electron source to generate a focused electron beam that interacts with the sample, generating signals that can be detected and processed to create images and gather analytical data.
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Lab products found in correlation
2 protocols using vega3 tungsten scanning electron microscope
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Microstructure Analysis of BNC and ENC
2
Scanning Electron Microscopy of Samples
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Scanning electron microscopy (SEM) was used to observe the microstructure of samples. The samples were fixed in 0.25% glutaraldehyde (Sigma) for 2 h and rinsed with PBS for 15 min three times. After dehydration through a graded series of ethanol, samples were critical-point dried with carbon dioxide. Afterwards, the samples were sputter coated with gold and observed under VEGA3 tungsten scanning electron microscope (TESCAN, Czech).
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