Genomic DNA was extracted using a DNeasy kit (Qiagen, Manchester, UK) which included RNase treatment (Qiagen). For RNA extraction tissue samples were homogenised in Trizol (Invitrogen) and total RNA extracted using a combination of a phenol-chloroform extraction and an RNeasy kit (Qiagen) which included DNase treatment (Qiagen). For cDNA synthesis 400 ng RNA was reverse transcribed using the Superscript II kit (Invitrogen) according to manufacturer’s protocol.
Rnase treatment
Manufactured by Qiagen
Sourced in United Kingdom
RNase treatment is a laboratory product designed to remove ribonucleic acid (RNA) from samples. It is used to eliminate potential RNA contamination in various experimental procedures.
Automatically generated - may contain errors
2 protocols using rnase treatment
1
Genomic and Transcriptomic DNA/RNA Extraction
2
Isolation of High-Molecular-Weight DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total high-molecular-weight DNA of transgenic and WT ES cells was isolated by standard DNA isolation protocol. This consists of lysis in 17.5 mM sodium dodecyl sulfate (SDS), 50 mM Tris (pH 8.0), 10 mM ethylenediaminetetraacetic acid (EDTA), proteinase K (200 µg/mL, Carl Roth); phenol-chloroform-isoamyl alcohol extraction (Sigma-Aldrich, Merck, 25:24:1); chloroform-isoamyl extraction (Sigma-Aldrich, Merck, 24:1); precipitation and resuspension of DNA in 10 mM Tris and 1 mM EDTA, pH 8.0 (TE); RNase treatment (1 mg/mL, Qiagen); further extraction; and precipitation and resuspension of DNA in TE buffer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!