Tebufenpyrad

All acaricides were technical grade and were obtained from the following sources: fenothiocarb (99.8%, Sigma Aldrich, Saint Louis, Mo) monocrotophos (99.5%, Chem Service, West Chester, PA), omethoate (97.0%, Sigma Aldrich), endosulfan (99.4%, Sigma Aldrich), bifenthrin (99.0%, Sigma Aldrich), fenpropathrin (99.5%, Chem Service, West Chester, PA), Abamectin (97.6%, Sigma Aldrich), etoxazole (98.5%, Sigma Aldrich), fenbutatin oxide (97.7%, Sigma Aldrich), chlorfenapyr (99.6%, Sigma Aldrich), flufenoxuron (98.4%, Sigma Aldrich), amitraz (96.8%, Sigma Aldrich), pyridaben (99.7%, Sigma Aldrich), tebufenpyrad (99.8%, Sigma Aldrich), spiromesifen (99.8%, Sigma Aldrich), (EZ)-cyenopyrafen (97.3%, Sigma Aldrich), cyflumetofen (96.0%, Wako Pure Chemical Industries, Ltd., Japan), bifenazate (99.9%, Sigma Aldrich) and dicofol (97.2%, Sigma Aldrich).

L-menthol (natural source, food grade, ≥ 99% purity), BHT (food grade, ≥ 99% purity), formic acid, elevated purity grade solvents (acetonitrile, ethanol), as well as analytical standards of azoxystrobin, boscalid, buprofezin, chlorpyrifos, chlorpyrifos-methyl, clofentezine, dodine, fludioxonil, hexythiazox, methoxyfenozide, myclobutanil, penconazole, propiconazole, pyraclostrobin, pyriproxyfen, pyridaben, spirotetramat, tebuconazole and tebufenpyrad were purchased from Sigma Aldrich-Merck S.r.l. (Milan, Italy). A Milli-Q Plus apparatus (Millipore, Bedford, MA, USA.) was used for obtaining ultrapure water.
Weighted amounts of the analytical standards (OhausDV215CD Discovery semi-micro and analytical balance, 81/210 g capacity, 0.01/0.1 mg readability, Ohaus Corporation, Pine Brook, NJ, USA) were dissolved in methanol or toluene (clofentezine and pyraclostrobin) in volumetric flasks, in order to obtain individual stock solutions at a concentration of 1 mg mL⁻¹.The last ones were diluted in methanol for preparing the multi-standard working solutions at 0.02, 0.5, 1.5, 2.5 and 4 ng μL⁻¹ used for the method validation.