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Ab267646

Manufactured by Abcam
Sourced in United Kingdom, United States

Ab267646 is a laboratory reagent that can be used for various research applications. It is a purified protein that serves as a specific molecular probe. The core function of this product is to facilitate the detection and analysis of target molecules in biological samples.

Automatically generated - may contain errors

2 protocols using ab267646

1

Fanconi Anemia Pathway Regulation

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CRA (98%) was purchased from Shanghai Acmec Biochemical Co., Ltd. (123-73-9, Shanghai, China), and HAECs were purchased from BeNa Culture Collection (BNCC337727; Beijing, China). Fanconi anemia complementation group A (FANCA) small interfering RNA (siRNA) and siRNA-Mate were synthesized and provided by GenePharma Co., Ltd. (G04002, Shanghai, China). Glutathione peroxidase (GSH-Px) assay kits were obtained from the Jiancheng Bioengineering Institute (A005; Nanjing, Jiangsu, China). Phospho-extracellular signal-regulated kinase (P-ERK, ab176660), phospho-c-Jun N-terminal kinase (P-JNK, ab176645), phospho-p38 kinase (ab221013), cyclooxygenase-2 (COX-2, ab267646), Wnt3a (ab54479), β-catenin (ab16051), phospho- ErbB2 (ab101229), and phospho-ErbB4 (ab61059) ELISA kits were purchased from Abcam (Cambridge, UK), and mitochondrial membrane potential assay kits were obtained from Abbkine Scientific Co., Ltd. (KTA4001; Wuhan, Hubei, China).
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2

Quantifying PGE2 and COX-2 in Cell Cultures

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The level of human Prostaglandin E2 (PGE2) and human cyclooxygenase-2 (COX-2) was estimated by an ELISA kit. The following ELISA kits were purchased from their respective sources: human PGE2 (KGE004B; R&D Systems, Minneapolis, MN, USA), human COX-2 (ab267646; Abcam). Moreover, 1 × 105 cells were cultured in media with DPB-LPS and/or EEAKSs for 24 h. The medium was used for analysis.
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