Mouse monoclonal antibody against vinculin

The effect of chiral hydrogels on the adhesive capacity of hDPSCs was evaluated. Briefly, hDPSCs (2 × 10⁴/mL) were incubated in confocal dishes containing different chiral nanofiber hydrogels and growth medium for 24 h. Cells were then rinsed with PBS, fixed with 4 % paraformaldehyde for 20 min, and permeabilised with 0.1 % Triton X-100 for 15 min. After blocking with 5 % bovine serum albumin for 30 min, the cells were incubated overnight at 4 °C with a mouse monoclonal antibody against vinculin (dilution 1:100; Sigma-Aldrich Corporation), followed by a secondary antibody (Dako, Glostrup, Denmark) for 1 h at room temperature. The cytoskeleton was stained with fluorescein isothiocyanate-labelled phalloidin (Yeasen, China) for 1 h, and nuclei were stained with 4′,6-diamidino-2-phenylindole for 5 min. Images were obtained using a confocal laser-scanning microscope (Leica Microsystems GmbH, Wetzlar, Germany).