The following commercially available antibodies were used for Western blotting: mouse monoclonal antibodies against clathrin heavy chain (CHC; BD Biosciences; 610500), lamin A/C (Santa Cruz Biotechnology; sc-7292), Hsp90 (Santa Cruz Biotechnology; sc-13119), EHD2 (Santa Cruz Biotechnology; sc-100724), dynamin (BD Biosciences; 610245), and filamin A (Chemicon; MAB1678); rabbit polyclonal antibodies against SUMO2/3 (Cell Signaling Technology; 4971), Cav1 (BD Biosciences; 610059), pacsin2 (Abgent; AP8088b); and cavin1 (Sigma; AV36965); for immunofluorescence, mouse monoclonal anti-cavin1 (BD Biosciences; 611258), goat polyclonal anti-EHD2 (Abcam; Ab23935), rabbit polyclonal anti-Cav1 (BD Biosciences; 610059), mouse monoclonal anti-lamin A/C (Santa Cruz Biotechnology; sc-7292), rabbit polyclonal anti-SUMO1 (Cell Signaling Technology; 4930), and rabbit polyclonal anti-SUMO2/3 (Cell Signaling Technology; 4971). Antibodies conjugated to Alexa Fluor 488, Cy3, Cy5, or HRP (Beckman Coulter and Invitrogen) were used as secondary antibodies. HaloTag dye JF635 was provided by L. Lavis (Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA). Accutase was purchased from Sigma-Aldrich. Hepes, SDS, and Tris were purchased from Euromedex.
Hepes
Manufactured by Euromedex
Hepes is a chemical compound commonly used as a buffer solution in cell and tissue culture applications. It is known for its ability to maintain a stable pH environment, which is crucial for the optimal growth and maintenance of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Accutase, by Merck Group (1 mentions)
Anti sumo1, by Cell Signaling Technology (1 mentions)
Anti cav1, by BD (1 mentions)
Anti sumo2 3, by Cell Signaling Technology (1 mentions)
Sodium dodecyl sulfate (sds), by Euromedex (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
2 protocols using hepes
1
Western Blotting and Immunofluorescence Antibodies
2
Culturing Virulent Theileria annulata-Transformed Macrophages
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Virulent Theileria annulata-transformed Ode macrophages (first isolated in Anand India) at passage 5220 (link) were cultured in RPMI that contained 10% FBS (Gibco), penicillin/streptomycin (Gibco), HEPES (Euromedex) and L-glutamine. The D7 cell line45 , bovine leukemia virus immortalized B cells (BL20)46 (link) and their T. annulata-transformed counterparts (TBL20, Hissar parasite strain)47 (link) were cultured under the same conditions except that 2-mercaptoethanol (Gibco) was added to the culture medium. Cultures were passaged 3-times per week.
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