All chemicals were purchased from Sigma Aldrich and used without additional purifications. Cyano-polyphenylene vinylene (CN-PPV, MW= 20 - 100 kDa) was purchased from American Dye Source, Inc. while polystyrene derivatives (PS-COOH, 50 kDa and PS-NH2, 6.5 kDa) was purchased from Polymer Source, Inc. Biotinylated Mouse IgG was purchased from Abcam, while Anti-IgG magnetic beads were purchased from New England BioLabs, Inc.
Anti igg magnetic beads
Manufactured by New England Biolabs
Anti-IgG magnetic beads are a type of laboratory equipment used for the isolation and purification of immunoglobulin G (IgG) from biological samples. They consist of a magnetic core coated with anti-IgG antibodies, which can bind to and capture IgG molecules present in the sample. These beads can be easily separated from the sample using a magnetic field, allowing for the efficient isolation of the target IgG.
Automatically generated - may contain errors
2 protocols using anti igg magnetic beads
1
Biomolecular Conjugation Synthesis
2
Studying Wnt Signaling Protein Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hek293t cells were co-transfected using linear PEI (1 mg/ml) with plasmids encoding HA-Gtpbp2, Myc, Myc-Gsk3b, and/or Myc-Axin. Cells were incubated overnight and lysed in NP40 lysis buffer (10 mM Tris-Cl pH 8.0, 137 mM NaCl, 10 % Glycerol, 1 % NP-40, + complete protease and phosphatase inhibitor tablets; Roche). Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions. Immunoprecipitations and whole cell lysates (1:10 of IP volumes) were blotted with mouse anti-myc (1:100) and rabbit anti-HA antibodies (1:500). For western blotting Xenopus embryos or explants were lysed in NP40 lysis buffer and extracted with an equal volume of 1,1,2-trichlorotrifluoro-ethane (Sigma), and then separated using SDS-page. Detection of western blots was conducted with IR secondary antibodies (Alexa 680 and IRdye800) scanned on a Licor Odyssey Classic Imager. Levels of ectopic myc-Axin were normalized to levels of co-injected HA-mCherry.
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