Trolox

The reagents included solasonine (HY-N0070, MCE), Z-VAD-FMK (S7023, Selleck), DCFH-DA (S0033S, Beyotime), Cell Counting Kit-8 (40203ES76, YEASEN), SYTOX green (S7020, Invitrogen), Trolox (C3183, APExBIO), DFO (HY-B0988, MCE), Mito-TEMPO (HY-112879, MCE), Necrostatin-1(HY-15760, MCE), Hoechst 3342 (23491-52-3, Solarbio), Ferrostatin-1 (HY-100579, MCE), RSL3 (HY-100218A, MCE), Bafilomycin A1 (HY-100558, MCE), C11-BODIPY581/591 (D3861, Invitrogen), Erastin (HY-15763,MCE), GSH assay kit (A006-2-1, Nanjing Jiancheng), Cysteine assay kit (BC185, Solarbio), FerroOrange probes (M489, Dojindo), MitoPeDPP (M466-5,Dojindo), and JC-1 (MT09-1, Dojindo).The following antibody purchased from Cell Signaling Technology was GAPDH (5174, CST), the others purchased from BOSTER included GPX4 (BM5231, BOSTER) and SLCA711 (BM5318, BOSTER). According to the manufacturer’s instructions, the drugs were initially dissolved in dimethylsulfoxide (DMSO) at a stock solution.

The SLC7A11, GPx4, ACSL4, COX2 and GAPDH primary antibodies and the HRP conjugated anti-rabbit IgG secondary antibody were purchased from ABclonal Technology (Wuhan, China). Acridine orange (AO) dye was purchased from Solarbio, (Beijing, China). C11-BODIPY^581/591 was purchased from Thermo Fisher Scientific (MA, USA). FerroOrange, Mito-FerroGreen and LiperFluo were purchased from Dojindo Molecular Technology (Japan). MitoTEMPO were purchased from Sigma (MO, USA). 2′,7′-dichlorodihydrouorescein diacetate (DCFH-DA), Mito-Tracker Red, GSH/GSSG assay kit and Lipid Peroxidation MDA Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China). Cell Counting Kit 8, Annexin V-FITC/propidium iodide assay kit, MitoSOX™ Red Mitochondrial Superoxide Indicator and Mito-Tracker Green were purchased from Yeasen Biotechnology (Shanghai, China). Ferrostain-1, Deferoxamine mesylate (DFO) and Trolox were purchased from APExBIO (TX, USA).