Cells with different treatments were seeded in the 3.5 cm cell culture dish (Corning, Corning, NY, USA) at the density of 150/dish (with 2 mL DMEM with 10% FBS). Then, 200 μL of DMEM with 10% FBS was added into the dish every 2 days to supplement the medium that volatilized into the incubator. Cells were incubated for 10 to 14 days until the colonies were visible to the naked eye on the bottom of the culture dish. After removing the medium, cell colonies were fixed with methanol for 30 minutes and then stained with 0.1% crystal violet (Solarbio, Beijing, China). The number of colonies stained in the dish for analysis was then counted, and the experiment was repeated three times.
3.5 cm cell culture dish
Manufactured by Corning
Sourced in United States, China
The 3.5 cm cell culture dish is a standard laboratory equipment used for the cultivation and study of cells in controlled environments. It provides a sterile, flat surface for cell growth and observation. The dish has a diameter of 3.5 cm, which is a common size for various cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 3.5 cm cell culture dish
1
Colony Formation Assay Protocol
2
Colony Forming Assay for Tumor Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 3.5 cm cell culture dish (Corning, Chengdu, Sichuan, China) was used to seeding the cells. The 200 μL DMEM containing 10% FBS, working as the medium that volatilized into the incubator, was supplemented into the dish every 2 days. After 2 weeks, the colonies were visible to the naked eye. Next, 95% methanol was used to fix the cells which were further stained by the use of methyl violet(C0089, Baomanbio, Xuhui, Shanghai, China). Under an IX71 inverted microscope, our group counted the tumor colonies (>50 cells).
3
Continuous Giant Unilamellar Vesicle Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rotating component
of the cDICE device is built from a magnetic stirrer L-71 (LAbinco).
A Teflon adaptor connects the rotating shaft with the cDICE chamber,
assembled from a standard 3.5 cm cell culture dish (corning) modified
to decrease the height. The chamber has an inner height of 8 mm and
a circular opening of 10 mm on the top to allow insertion of the capillary
and collection of the GUVs. The detailed protocol for GUV production
is reported in Van de Cauter et al.,22 (link) while here we describe it briefly. First, the cDICE chamber
is set to rotate at 300 rpm. Next, 350 μL of outer aqueous solution
is injected into the rotating the chamber, followed by 1 mL of lipid-in-oil
solution, resulting in the two phases being stacked, as depicted inFigure 3 a. The tip of a 100
μm PEEK capillary tube (211633-3, BGB) is then inserted into
the oil phase of the rotating chamber, allowing for a continuous supply
of inner aqueous solution into the oil phase by a pressure pump (MFCS-EZ,
Fluigent) set at 900 mbar. After 15 min, the rotating speed is set
to zero, and GUVs are collected from the outer aqueous solution and
moved to a prepassivated imaging well to carry out the fluorescence
experiments.
of the cDICE device is built from a magnetic stirrer L-71 (LAbinco).
A Teflon adaptor connects the rotating shaft with the cDICE chamber,
assembled from a standard 3.5 cm cell culture dish (corning) modified
to decrease the height. The chamber has an inner height of 8 mm and
a circular opening of 10 mm on the top to allow insertion of the capillary
and collection of the GUVs. The detailed protocol for GUV production
is reported in Van de Cauter et al.,22 (link) while here we describe it briefly. First, the cDICE chamber
is set to rotate at 300 rpm. Next, 350 μL of outer aqueous solution
is injected into the rotating the chamber, followed by 1 mL of lipid-in-oil
solution, resulting in the two phases being stacked, as depicted in
μm PEEK capillary tube (211633-3, BGB) is then inserted into
the oil phase of the rotating chamber, allowing for a continuous supply
of inner aqueous solution into the oil phase by a pressure pump (MFCS-EZ,
Fluigent) set at 900 mbar. After 15 min, the rotating speed is set
to zero, and GUVs are collected from the outer aqueous solution and
moved to a prepassivated imaging well to carry out the fluorescence
experiments.
4
Continuous Formation of Giant Unilamellar Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rotating component of the cDICE device is built from a Magnetic stirrer L-71 (LAbinco).
A Teflon adaptor connects the rotating shaft with the cDICE chamber, assembled from a standard 3.5 cm cell culture dish (corning) modified to decrease the height. The chamber has an inner height of 8 mm and a circular opening of 10 mm on the top to allow insertion of the capillary and collection of the GUVs. The detailed protocol for GUV production is reported in Van de Cauter et al. 22 , while here we describe it briefly. First, the cDICE chamber is set to rotate at 300 rpm. 350 µL of outer aqueous solution is injected into the rotating the chamber, followed by 1 mL of lipid-in-oil solution, resulting in the two phases being stacked as depicted in Fig. 3a. The tip of a 100 µm PEEK capillary tube (211633-3, BGB) is then inserted into the oil phase of the rotating chamber, allowing for a continuous supply of inner aqueous solution into the oil phase by a pressure pump (MFCS-EZ, Fluigent) set at 900 mbar. After 15 min, the rotating speed is set to zero, and GUVs are collected from the outer aqueous solution and moved to a pre-passivated imaging well to carry out the fluorescence experiments.
A Teflon adaptor connects the rotating shaft with the cDICE chamber, assembled from a standard 3.5 cm cell culture dish (corning) modified to decrease the height. The chamber has an inner height of 8 mm and a circular opening of 10 mm on the top to allow insertion of the capillary and collection of the GUVs. The detailed protocol for GUV production is reported in Van de Cauter et al. 22 , while here we describe it briefly. First, the cDICE chamber is set to rotate at 300 rpm. 350 µL of outer aqueous solution is injected into the rotating the chamber, followed by 1 mL of lipid-in-oil solution, resulting in the two phases being stacked as depicted in Fig. 3a. The tip of a 100 µm PEEK capillary tube (211633-3, BGB) is then inserted into the oil phase of the rotating chamber, allowing for a continuous supply of inner aqueous solution into the oil phase by a pressure pump (MFCS-EZ, Fluigent) set at 900 mbar. After 15 min, the rotating speed is set to zero, and GUVs are collected from the outer aqueous solution and moved to a pre-passivated imaging well to carry out the fluorescence experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!