Anti cd154 clone 24 31

Manufactured by BioLegend

Sourced in United States

The Anti-CD154 (clone 24-31) is a lab equipment product manufactured by BioLegend. It is a monoclonal antibody that binds to the CD154 (CD40 ligand) molecule.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cd69 clone fn50, by BioLegend (1 mentions) Anti ki 67 clone b56, by BD (1 mentions) Aim 5 medium, by Thermo Fisher Scientific (1 mentions) Anti tnf α clone mab11, by BioLegend (1 mentions) Anti il2 clone mq1 17h12, by BioLegend (1 mentions) Facs symphony a3 analyzer, by BD (1 mentions)

Spelling variants of this product

CD154 (4 citations)

2 protocols using anti cd154 clone 24 31

Activation-Induced Marker (AIM) Staining of Antigen-Specific T Cells

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After peptide stimulation, the cells were washed with PBS containing 5% FCS and stained with amine-reactive viability stain (Live/dead fixable aqua dead cell stain kit; Invitrogen) for 30 min at 4°C. After washing, pMHC-spheromers were added to screen the epitope-specific CD8⁺ and CD4⁺ T cells. Meanwhile, the antibody cocktail was added for AIM staining (anti-CD69 (clone FN50, Biolegend), anti-CD154 (clone 24-31, Biolegend), anti-CD137 (clone 4B4-1, Biolegend), anti-CD38 (clone HIT2, BD Biosciences) and anti-Ki-67 (clone B56, BD Biosciences)). The cells were stained for 30 min at 4°C in 100μl volume.
Cells were then washed twice with staining buffer before acquisition using BD LSRII flow cytometer. The data was analyzed using FlowJo (v10) software.

Gao F., Mallajoysula V., Arunachalam P.S., van der Ploeg K., Manohar M., Röltgen K., Yang F., Wirz O., Hoh R., Haraguchi E., Lee J.Y., Willis R., Ramachandiran V., Li J., Kathuria K.R., Li C., Lee A.S., Shah M.M., Sindher S.B., Gonzalez J., Altman J.D., Wang T.T., Boyd S.D., Pulendran B., Jagannathan P., Nadeau K.C, & Davis M.M. (2023). Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection. Immunity, 56(4), 864-878.e4.

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Characterizing CD4+ T Cell Responses to SARS-CoV-2 Variants

Cited 1 time

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T cell lines were plated at a concentration of 25 × 10⁴ cells/well in 100 μL AIM-V medium (ThermoFisher, Waltham, MA, USA) with 2% human serum in 96-wells plate, and restimulated with either “WT CD4⁺ pool” or “Omicron BA.4/BA.5 CD4⁺ pool” (1 μM/peptide) for 6 h at 37 °C, 5% CO₂. Cells were washed and stained as previously described [22 (link)] with minor modifications as indicated.
Cells were stained intracellularly for anti-CD154 (clone 24–31; Biolegend, San Diego, CA, USA), and anti-IFN-ɣ (clone 4S.B3; BD Bioscience, Allschwil, Switzerland), anti-IL-2 (clone MQ1-17H12; Biolegend), anti-TNF-α (clone Mab11; Biolegend, San Diego, CA, USA), anti-IL-5 (clone TRFK5) and anti-IL-13 (clone JES10-5A2; Both BD Biosciences, Allschwil, Switzerland). Approximately 133,000 events were acquired on a FACS Symphony A3 analyzer (BD Biosciences, Allschwil, Switzerland). FlowJo (version 10, Tree Star, Ashland, OR, USA) was used for flow cytometry data analysis.

Emmelot M.E., Vos M., Boer M.C., Rots N.Y., van Els C.A, & Kaaijk P. (2022). SARS-CoV-2 Omicron BA.4/BA.5 Mutations in Spike Leading to T Cell Escape in Recently Vaccinated Individuals. Viruses, 15(1), 101.

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