L-Glutathione reduced (GSH), hydrochloric acid (HCl), sodium nitrite, and polyethylene glycol (MW = 3,350) were purchased from Sigma-Aldrich (St. Louis, MO). Acetone; DOWSIL 3140, MIL-A-46146 RTV Silicone Coating; Dow Corning Silastic Laboratory Tubing (ID 0.058" × OD 0.077", 2415542); and Helix Medical Inc. Silicone Tubing (0.125" × 0.250", 6001121) were purchased from Fisher Scientific Inc. (Pittsburgh, PA). Male Luer Lock Injection Site caps (80149) were purchased from Qosina (Ronkonkoma, NY). Luria-Bertani (LB) agar broth and 10 mM phosphate buffered saline (PBS) (pH 7.2) were purchased from ThermoFisher Scientific (Grand Island, NY). Zinc oxide nanoparticles (APS 30 nm in diameter) were purchased from EPRUI Biotech Co. Ltd. (ShangHai, China). All aqueous solutions were prepared with 18.2 M Ω deionized water using a Milli-Q filter (Milli-q purified water) from EMD Millipore (Billerica, MA). Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 were obtained from the American Type Culture Collection (Manassas, VA).
Silicone tubing
Manufactured by Thermo Fisher Scientific
Sourced in United States
Silicone Tubing is a flexible and durable hose made of food-grade silicone material. It is designed to transfer liquids and gases in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyethylene glycol, by Merck Group (1 mentions)
Staphylococcus aureus atcc 25923, by American Type Culture Collection (1 mentions)
Pseudomonas aeruginosa atcc 27853, by American Type Culture Collection (1 mentions)
Milli q filter, by Merck Group (1 mentions)
L glutathione reduced gsh, by Merck Group (1 mentions)
Vls 6, by Universal Systems (1 mentions)
Solidworks cad, by Dassault Systèmes (1 mentions)
Em ccd camera, by Hamamatsu Photonics (1 mentions)
Axiovert microscope, by Zeiss (1 mentions)
Axiovision software, by Zeiss (1 mentions)
3 protocols using silicone tubing
1
Synthesis and Characterization of Antimicrobial Nanocomposites
2
Fabrication of PMMA Microfluidic Devices
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The microfluidic devices were designed using Solidworks CAD (version 24, Dassault Systems, Vélizy-Villacoublay, France) software. A computer numerical control (CNC) program was then created using Mastercam (CNC Software Inc., Tolland, CT, USA), according to the microfluidic circuit design and dimensions.
The outline of the device was extracted from a PMMA plastic sheet (MacMaster-Carr) using a Laser engraver (Universal Laser Systems, VLS 6.30, Scottsdale, AZ, USA). The plastic cut out was then taken to a CNC machine (HAAS Super Minimill), where the microfluidic circuit was created based on the Mastercam program. Milling conditions: milling direction, spindle speed, feed rate were optimized for surface quality. Finally, fluidic ports were created using a drill press (Ellis).
The PMMA device was sealed with a self-adhesive pouch (Opko diagnosis). Main channel dimensions are displayed inFigure 1 The device was connected to open barrels of 10 mL disposable plastic syringes (BD biosciences) via silicone tubing (ID 1.1 mm OD 2.16 mm) (Fisher scientific, Waltham, MA, USA). The driving force used for the experiments was gravity, so liquid pressure on the microdevice was controlled by the heights of the open syringes. Pieces of polytetrafluoroethylene (PTFE) tubing (OD 2 mm) (Cole Parmer, Vernon Hills, IL, USA) were used to interface the reservoirs to the chip.
The outline of the device was extracted from a PMMA plastic sheet (MacMaster-Carr) using a Laser engraver (Universal Laser Systems, VLS 6.30, Scottsdale, AZ, USA). The plastic cut out was then taken to a CNC machine (HAAS Super Minimill), where the microfluidic circuit was created based on the Mastercam program. Milling conditions: milling direction, spindle speed, feed rate were optimized for surface quality. Finally, fluidic ports were created using a drill press (Ellis).
The PMMA device was sealed with a self-adhesive pouch (Opko diagnosis). Main channel dimensions are displayed in
3
Live Cell Imaging of Organelles
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