P dimethylamino cinnamaldehyde p dmaca

Lipase from porcine pancreatic (Type II, EC 3.1.1.3; L3126), α‐amylase from porcine pancreas (Type VI‐B, EC 3.2.1.1, A3176), α‐glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20, G3651), and acetylcholinesterase from Electrophorus electricus (Type VI‐S, EC 3.1.1.7, C3389) were purchased from Sigma‐Aldrich Co., Ltd. Rutin (≥98%), ascorbic acid (≥98%), p‐nitrophenyl palmitate (p‐NPP), 3,5‐dinitrosalicylic acid (DNS), p‐nitrophenyl‐α‐D‐glucopyranoside (α‐p‐NPG), 5,5'‐dithiobis(2‐nitrobenzoic acid) (DTNB), acetylthiocholine iodide, and orlistat were purchased from Hefei Biobomei Biotechnoligy Co., Ltd.. Acarbose was purchased from Nanjing Dulai Biotechnology Co., Ltd.. p‐(Dimethylamino)cinnamaldehyde (p‐DMACA) and galantamine were purchased from Shanghai Yuanye Biotechnology Co., Ltd.. All other chemicals used were of analytical grade and procured from Sinopharm Chemical Reagent Co., Ltd. AB‐8 macroporous resin was purchased from Anhui Sanxing Resin Technology Co., Ltd.

Gallic acid (≥98%), rutin (≥98%), catechin (≥98%), pyrogallol (≥98%), ascorbic acid, p-nitrophenyl palmitate (p-NPP), orlistat, 3,5-dinitrosalicylic acid (DNS), p-nitrophenyl-α-D-glucopyranoside (p-NPG), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), and acetylthiocholine iodide (AChI) were purchased from Hefei Bomei Biotechnology Co., Ltd. (Hefei, Anhui, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, M5655, ≥97.5%) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Folin–Ciocalteau reagent was purchased from Shanghai Jinsui Biotechnology Co., Ltd. (Shanghai, China). p-(dimethylamino)cinnamaldehyde (p-DMACA) and galanthamine were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The human cervical carcinoma cell line HeLa, the human liver cancer cell line HepG2, and human large cell lung cancer cell line NCI-H460 were obtained from the Cell bank of typical culture preservation Committee of the Chinese Academy of Sciences. Trypsin–EDTA solution and 5-FU (F6627, ≥99%) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). All other chemicals used were of analytical grade and procured from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). AB-8 macroporous resin was purchased from Anhui Sanxing Resin Technology Co., Ltd. (Bengbu, Anhui, China).