L type wako j

Total LDH activity (IU/l) was measured by an autoanalyzer JCA-BM6050 (JEOL, Tokyo, Japan) using enzymatic method L-Type Wako LD IF or L-Type Wako J (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). The percentages of LDH isozymes were measured using a Hydrasys 2 Scan (Sebia, Paris, France) with a HYDRAGEL 7 ISO-LDH (Sebia).
Measurements of total LDH activity and LDH isozymes were performed by a clinical laboratory testing company, Fujifilm Vet Systems (Tokyo, Japan).

A previous study indicated that the total LDH isozyme activity in blood reflected the disease progression of EBL; in particular, increased percentages of the LDH 2+3 isozyme ratio were established as a key parameter for the diagnosis of lymphosarcoma [28 (link)]. Therefore, the current study also focused on the total LDH activity and LDH isozyme ratio in blood from BLV-infected cattle with LPVL and HPVL. The total LDH activity (IU/L) was measured using an auto analyzer JCS-BM6050 (JEOL, Tokyo, Japan) using an enzymatic method (L-Type Wako LD IF or L-Type Wako J, Fujifilm Wako Pure Chemical, Osaka, Japan). The percentage of the LDH isozyme ratio was measured by a Hydrasys 2 Scan (Sebia, Paris, France) using HYDRAGEL 7 ISO-LDH (Sebia), and was conducted by a clinical laboratory testing company, Fujifilm Vet Systems (Tokyo, Japan).