5µm sections of formalin fixed paraffin embedded tissue were deparaffinized and washed in gradient ethanol 100%, 90%, 70% and water. After antigen retrieval (boiling at 95°C for 20 minutes) in Dako modified citrate-based buffer (Dako, S1700), sections were blocked with 3% BSA in PBS-Triton (0.03% v/v) overnight, and then incubated with anti-EpCAM antibody (Santa Cruz Biotechnology, Cat# sc53532), anti-iNOS antibody (Abcam, Cat#: ab15323), F4/80 antibody (Abcam, Cat# ab6640), anti-nitrotyrosine antibody (Millipore, Cat#: 05-233), anti-Ki67 antibody (BD Pharmingen, Cat#: 550809), anti-γH2AX antibody (Cell signaling, Cat#: 9718), anti-Phospho-STAT3 (Tyr705) (Cell Signaling, Cat#: 9145), and anti-MPO antibody (Abcam cat#: ab45977) at room temperature for one hour. Primary antibodies were visualized with Alexa-Fluor-568 conjugated or Alexa-Fluor-488 conjugated secondary antibodies (goat-anti-rabbit, goat-anti-rat, or goat-anti-mouse, Invitrogen). Tissue sections were counter-stained with DAPI. All the stained slides were examined under a Zeiss Axioskop 2 plus microscope with QIClick digital CCD Camera (QImaging). Morphometric analysis from at least 8 random images was performed using Image Pro-Plus (version 7.2, Media Cybematics, Silver Spring, MD, USA). All images were acquired using a 20X or 100X objective lens.
Anti epcam antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-EpCAM antibody is a primary antibody that specifically binds to the Epithelial Cell Adhesion Molecule (EpCAM). EpCAM is a cell surface protein that is expressed on epithelial cells and some cancer cells. This antibody can be used for the detection and analysis of EpCAM in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ki67 antibody, by BD (2 mentions)
Anti γh2ax antibody, by Cell Signaling Technology (2 mentions)
F4 80 antibody, by Abcam (2 mentions)
Anti phospho stat3 tyr705, by Cell Signaling Technology (2 mentions)
Anti nitrotyrosine antibody, by Merck Group (2 mentions)
Anti mpo antibody, by Abcam (2 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Anti inos antibody, by Abcam (2 mentions)
Anti cd133, by Abcam (1 mentions)
Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti phospho mtor, by Cell Signaling Technology (1 mentions)
Anti phosphor ampk, by Cell Signaling Technology (1 mentions)
Anti mtor, by Cell Signaling Technology (1 mentions)
Anti cd44 antibody, by Abcam (1 mentions)
Anti ampk, by Cell Signaling Technology (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti epcam antibody
1
Multimarker Immunofluorescence Analysis
2
Multimarker Immunofluorescence Analysis
3
Molecular Mechanisms of Stem Cell Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hepatocyte growth factor (HGF) and transforming growth factor β 1 (TGF-β-1) were purchased from Pepro Tech (Rocky Hill, USA). Rapamycin and AICAR were purchased from Calbiochem (San Diego, CA, USA). The anti-CD90 conjugated PE antibody was purchased from eBioscience (San Diego, CA, USA). The anti-EpCAM antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-mTOR and anti-CD90 antibodies were purchased from Epitomics (Burlingame, CA, USA). The anti-CD133 and anti-CD44 antibodies were purchased from Abcam (Cambridge, UK). The anti-phospho-mTOR, anti-phosphor-AMPK, anti-AMPK, anti-mTOR, secondary horse anti-mouse horseradish peroxidase-conjugated and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Cell Signaling (Beverly, MA, USA). The anti-β-actin antibody was purchased from Chemicon (Pittsburgh, PA, USA). The AC133 antibody was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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