hTERT-immortalized human mammary epithelial cells (IMEC) and XD cells were cultured at 37 °C and 5% CO2 in 1:1 DMEM/F-12 medium (gibco #11320-074) supplemented with insulin (Clonetics, MEGM SingleQuots #CC-4136), EGF (Clonetics, MEGM SingleQuots #CC-4136), bovine pituitary extract (Clonetics, MEGM SingleQuots #CC-4136), hydrocortisone (Clonetics, MEGM SingleQuots #CC-4136) and 100 ng/ml cholera toxin (Sigma #8052). IMEC-MYC, IMEC-PIK3CAH1047R, IMEC-P53DD, and IMEC-RAS were generated by transducing IMEC with pMXs-c-Myc, PGK-PIK3CAH1047R, pBABE-puro-RAS V12, and MSCV-p53DD-iGFP vector, respectively. IMEC-MYC-7TGP cells were generated by transduction of IMEC-MYC with 7TGP vector. T47D and MCF7 cells were cultured at 37 °C and 5% CO2 in DMEM high glucose (Euroclone #ECB7501L) supplemented with 10% fetal bovine serum (Euroclone #ECS0180L), 1 mM sodium pyruvate (Euroclone #ECM0542D) and 2 mM glutamine (Euroclone #ECB3000D). ZR751 cells were cultured at 37 °C and 5% CO2 in RMPI Medium 1640 (gibco #31870-025) supplemented with 10% fetal bovine serum, 2 mM glutamine and 1 mM sodium pyruvate. MCF7-MYC, T47D-MYC, and ZR751-MYC were generated by transduction with pMXs-c-Myc.
Poli V., Fagnocchi L., Fasciani A., Cherubini A., Mazzoleni S., Ferrillo S., Miluzio A., Gaudioso G., Vaira V., Turdo A., Gaggianesi M., Chinnici A., Lipari E., Bicciato S., Bosari S., Todaro M, & Zippo A. (2018). MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state. Nature Communications, 9, 1024.
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