Rnadvance magnetic beads

Total RNA was extracted with the RNeasy Kit (Qiagen). After DNase I treatment, 3 ug of total RNA was used to synthesize the cDNA using iScript™ Advanced cDNA Synthesis Kit (Bio-Rad). cDNA was purified with MinElute PCR Purification Kit (Qiagen).
For the ADAR EA mutant RNAseq, total RNA was extracted using RNAdvance magnetic beads (Agencourt), treated using TURBO DNase (Thermo Fisher Scientific), depleted of ribosomal RNA [50 (link)], and treated again using TURBO DNase.

All stocks were grown on standard fly media. Adar^5G1 (“Adar null”) flies are from^{31 (link)}. The Cha-Gal4 driver expresses GAL4 in cholinergic neurons under the control of the choline acetyl transferase promoter (Cha is now named ChAT in Flybase http://flybase.org/reports/FBti0024050.html); this GAL4 driver line is Bloomington Stock number 6798, w1118; P^{21 (link),48 (link)}s19B/CyO, P{sevRas1.V12}FK1. The UAS-Adar 3/4 transgene expresses an editable cDNA encoding the adult ADAR 3/4 splice form^{30 (link)}. The UAS-hADAR2 transgene expresses cDNA encoding human ADAR2. RNAi lines  are Adar (VDRC-7763), Dcr-2 (VDRC-25090), Rel (VDRC-105491), Dif (Bloomington-29514), Stat92E (Bloomington-35600). For the innate immune qPCR, flies were grown at 25 °C and collected at 2-3 days old. Total RNA was extracted from 7–15 whole flies or 20 heads using RNAdvance magnetic beads (Agencourt), and this was treated using TURBO DNase (Thermo Fisher Scientific).
For the locomotion assays (negative geotaxis and open field locomotion), flies were kept in 12 h/12 h light/dark cycles at 25 °C. Replicate experiments were performed at the same time of day. Flies were not exposed to carbon dioxide for at least 24 h before the assays.