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Cap system

Manufactured by Thermo Fisher Scientific
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The CAP system is a laboratory equipment designed for automated cell culture processes. It functions to provide a controlled and monitored environment for cell growth and maintenance.

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Lab products found in correlation

Maxm a l system, by Beckman Coulter (1 mentions) Enzyme linked immunosorbent assay, by R&D Systems (1 mentions) Niox mino, by NIOX Group (1 mentions)

8 protocols using cap system

1

IgE Measurement Using CAP System

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Total IgE and specific IgE were determined using the CAP system (Phadia, Uppsala, Sweden).
Probst M., Gogolka A., Krüll M, & Noga O. (2019). In search of clinically relevant parameters to monitor successful omalizumab therapy in allergic asthma. Allergologie Select, 2(1), 49-55.
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2

Lung Function and Allergy Biomarkers

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The percentages of predicted FEV 1 were measured using a spirometer (FUKUDA-77, Fukuda Denshi), and the best of 3 expirations was recorded. The predicted values of FEV 1 were calculated using published equations [17, 18] . Eosinophils in peripheral blood were counted automatically using a counter (Beckman Coulter) and the MAXM A/L system (Beckman Coulter). Serum levels of total IgE were measured using the CAP system (Phadia), and the plasma concentration of TARC was assessed using enzyme-linked immunosorbent assay (R&D Systems), as reported elsewhere [19] .
Kurosawa M, & Sutoh E. (2019). Prospective Open-Label Study of 48-Week Subcutaneous Administration of Mepolizumab in Japanese Patients With Severe Eosinophilic Asthma. Journal of investigational allergology & clinical immunology, 29(1).
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3

Allergy Patients Characterized for PFS

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Patients with birch pollen allergy and concomitant PFS were selected based on case history, positive skin prick test responses, and/or in vitro IgE detection (CAP System; Thermo Fisher Scientific, Phadia AB, Uppsala, Sweden; see Table E1 in this article’s Online Repository at www.jacionline.org). Inclusion criteria were a CAP class of greater than 3 to birch and greater than 1 to apple and hazelnut. Experiments with patients’ sera were approved by the Ethics Committee of the University of Vienna (EK028/2006) and Salzburg (415-E/1398/4-2011). Written informed consent was obtained from all subjects included in the study.
Hofer H., Asam C., Hauser M., Nagl B., Laimer J., Himly M., Briza P., Ebner C., Lang R., Hawranek T., Bohle B., Lackner P., Ferreira F, & Wallner M. (2016). Tackling Bet v 1 and associated food allergies with a single hybrid protein. The Journal of allergy and clinical immunology, 140(2), 525-533.e10.
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4

Allergy Patients Characterized for PFS

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Patients with birch pollen allergy and concomitant PFS were selected based on case history, positive skin prick test responses, and/or in vitro IgE detection (CAP System; Thermo Fisher Scientific, Phadia AB, Uppsala, Sweden; see Table E1 in this article’s Online Repository at www.jacionline.org). Inclusion criteria were a CAP class of greater than 3 to birch and greater than 1 to apple and hazelnut. Experiments with patients’ sera were approved by the Ethics Committee of the University of Vienna (EK028/2006) and Salzburg (415-E/1398/4-2011). Written informed consent was obtained from all subjects included in the study.
Hofer H., Asam C., Hauser M., Nagl B., Laimer J., Himly M., Briza P., Ebner C., Lang R., Hawranek T., Bohle B., Lackner P., Ferreira F, & Wallner M. (2016). Tackling Bet v 1 and associated food allergies with a single hybrid protein. The Journal of allergy and clinical immunology, 140(2), 525-533.e10.
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5

Allergen-specific IgE Measurement

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Total IgE and specific IgE to house dust mite, cat, timothy grass, and Cladosporium herbarum (mould) in serum samples were measured in a central laboratory by the CAP system (Thermo Fisher, Uppsala, Sweden), as described in detail elsewhere 26. Positive specific IgE was defined by IgE ≥ 0.35 kU/L to at least one of the four allergens.
Bertelsen R.J., Rava M., Carsin A.E., Accordini S., Benediktsdóttir B., Dratva J., Franklin K.A., Heinrich J., Holm M., Janson C., Johannessen A., Jarvis D.L., Jogi R., Leynaert B., Norback D., Omenaas E.R., Raherison C., Sánchez‐Ramos J.L., Schlünssen V., Sigsgaard T., Dharmage S.C, & Svanes C. (2017). Clinical markers of asthma and IgE assessed in parents before conception predict asthma and hayfever in the offspring. Clinical and Experimental Allergy, 47(5), 627-638.
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6

Birch Pollen Allergy Diagnostics

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Upon case history, positive in vivo skin prick test and in vitro IgE detection (CAP system, Thermo Fisher Scientific, Phadia AB, Uppsala, Uppland, Sweden), birch pollen allergic patients reactive to Bet v 2 were selected (n = 7). Informed consent was obtained from all subjects included in the study and experiments with patients’ sera were approved by the ethics committee of the Medical University of Vienna (no. EK028/2006).
Soh W.T., Briza P., Dall E., Asam C., Schubert M., Huber S., Aglas L., Bohle B., Ferreira F, & Brandstetter H. (2017). Two Distinct Conformations in Bet v 2 Determine Its Proteolytic Resistance to Cathepsin S. International Journal of Molecular Sciences, 18(10), 2156.
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7

Allergy Biomarkers Detection Protocol

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The levels of specific IgE to U. botrytis and Alt a 1 were measured using the CAP system (ThermoFisher Scientific, last Phadia, Uppsala, Sweden). The levels ≥0.35 kU/L were considered positive. The binding capacity for specific IgE protein extracts of C. albicans, U. botrytis, Mucor mucedo, Fusarium sp, Trichophyton rubrum, Aspergillus niger, A. alternata , S. chartarum, S. botryosum, P. notatum, Rhizopus sp, A. fumigatus, Botrytis sp, and C. herbarum were determined by IgE immunoblotting and Dot blot analysis.
Histamine release was made after stimulating blood basophils with the extracts of U. botrytis and Alt a 1 coupled to the CM-strip of the histamine release test (Reflab, Denmark) according to the method described by Per Stahl Skov et al.15 (link),16 (link)
Moreno A., Pineda F., Alcover J., Rodríguez D., Palacios R, & Martínez-Naves E. (2016). Orthologous Allergens and Diagnostic Utility of Major Allergen Alt a 1. Allergy, Asthma & Immunology Research, 8(5), 428-437.
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8

Proteomic and Biomarker Analysis of Sputum

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The SOMAscan proteomic assay of sputum supernatants performed by SomaLogic (Boulder, CO, USA) was used to obtain 1129 analytes [10] . Serum periostin was measured using a proprietary sandwich ELISA with two monoclonal antibodies capable of detecting all known splice variants of human periostin [11] . Serum or plasma cytokines IL-1α, IL-13, IL-17A, CCL11, CCL18 and CCL26 were assayed using ELISA techniques. FeNO was measured using an electrochemical analyser (NIOX MINO; Aerocrine, Solna, Sweden) at an expiratory flow rate of 50 mL•s -1 . Serum IgE was measured using the Thermo Fisher (Uppsala, Sweden) CAP system.
Pavlidis S., Takahashi K., Ng Kee Kwong F., Xie J., Hoda U., Sun K., Elyasigomari V., Agapow P., Loza M., Baribaud F., Chanez P., Fowler S.J., Shaw D.E., Fleming L.J., Howarth P.H., Sousa A.R., Corfield J., Auffray C., De Meulder B., Knowles R., Sterk P.J., Guo Y., Adcock I.M., Djukanovic R, & Fan Chung K. (2019). "T2-high" in severe asthma related to blood eosinophil, exhaled nitric oxide and serum periostin. The European respiratory journal, 53(1).
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