Ab73895

Manufactured by Abcam

Sourced in United Kingdom

Ab73895 is a product manufactured by Abcam. It is a lab equipment item, but a detailed description is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

3 protocols using ab73895

Measuring Angiogenic Factors in Samples

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The following reagents were employed: mouse anti-TSP1 clone A6.1 (Pierce, Alcobendas, Spain), TSP1 in human samples was detected with monoclonal anti-TSP1 ab1823 (Abcam, Cambridge, UK), HIF-2α was detected with anti-HIF-2α ab199 (mice) or ab73895 (human; Abcam), and HIF-1α was detected with polyclonal anti-HIF-1α C-term (Cayman Chemical Company, Ann Arbor, MI, USA), or monoclonal anti-HIF-1-a (610958, BD Biosciences, human samples) anti-Vinculin hVIN-1 (Sigma-Aldrich, Tres Cantos, Spain), anti-α-Tubulin T6199 (Sigma-Aldrich), anti-β-Actin (Cell Signaling Technology, Danvers, MA, USA), and rabbit anti-ZO-1 (Thermo Fisher Scientific, Alcobendas, Spain). Secondary antibodies were anti-IgG + IgM of mouse and rabbit conjugated with Peroxidase (Pierce), as well as goat anti-rabbit and goat anti-mouse antibodies conjugated with Alexa Fluor 488 (Invitrogen, Alcobendas, Spain). Alexa Fluor 568 phalloidin (Life Technologies, Alcobendas, Spain). TSP1 from human platelets was obtained from (Athens Research and Technology, Athens, GA, USA).

Labrousse-Arias D., Castillo-González R., Rogers N.M., Torres-Capelli M., Barreira B., Aragonés J., Cogolludo Á., Isenberg J.S, & Calzada M.J. (2015). HIF-2α-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction. Cardiovascular Research, 109(1), 115-130.

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Antibody-based Protein Expression Analysis

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All antibodies were commercially produced, tested, and recommended for the application. The primary capture antibodies in the ELISA analyses were rabbit anti-human ferroportin peptide (ab58695; Abcam, Cambridge, UK), rabbit anti-human IRP2 (LS-C80347, LifeSpan Biosciences, Seattle, WA, USA), rabbit anti-human HIF2 (ab73895; Abcam, Cambridge, UK). Mouse anti-human Immunoglobin G (IgG) conjugated with horseradish peroxidase (HRP; #55788; BD, Franklin Lakes, NJ, USA) was used as secondary capture antibody. The primary antibody used in the Western blot analyses was a rabbit anti-human ferroportin (NBP1-21502; Novus Biologicals, Littleton, MA, USA). A rabbit anti-human β-actin antibody (ab8227; Abcam, Cambridge, UK) was used as loading control. For Western blot analyses, the secondary antibody was goat anti-rabbit IgG conjugated to HRP (Bio-Rad, Sundbyberg, Sweden). Primary and secondary antibodies were used at 1 μg/mL.

Scheers N, & Sandberg A.S. (2014). Iron Transport through Ferroportin Is Induced by Intracellular Ascorbate and Involves IRP2 and HIF2α. Nutrients, 6(1), 249-260.

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Immunohistochemical Profiling of FFPE Tumor Samples

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Formalin-fixed paraffin embedded (FFPE) tumor tissue and RG cell pellets generated in our previously published study [8 ] were used for histological and immunohistochemical analyses. At least two slides (5μm thick) from each FFPE tumor sample were used for the analysis of each antibody presented in this study using standard immunohistochemical methods (see S2 Table).The immunohistochemical panel comprised the following antibodies: Anti-Ki-67 (RM-9106, Rabbit monoclonal, 1:200, Thermo scientific), POU5F1/OCT3/4 (LS-B85, Rabbit polyclonal, 1:300, LSBio), Anti-Nestin (ab105389, Rabbit monoclonal, 1:30, abcam), Anti- Sox2 (ab97959, Rabbit polyclonal, 1:200, abcam), Anti-Vimentin (NBP1-97671, Mouse monoclonal, 1:500, Novus Biologicals), Anti-c-MYC (ab32072, Rabbit monoclonal[Y69], 1:500, abcam), Anti-c-MYC-(Phospho S62) (ab185656, Rabbit monoclonal, 1:500, abcam), Anti-MAX (ab101271, Rabbit polyclonal, 1:1000, abcam), Anti-HIF-1α (ab82832, Rabbit polyclonal, 1:100, abcam), Anti-HIF-2α (ab73895, Rabbit polyclonal, 1:250, abcam), Anti-p53 (LS-B7722, Rabbit polyclonal, 1:200, LSBio), Anti-YB1 (ab12148, Rabbit polyclonal, 1:750, abcam), Anti-MDM2 (LS-C199239, Rabbit polyclonal, 1:100, LS Bio).

Malchenko S., Sredni S.T., Bi Y., Margaryan N.V., Boyineni J., Mohanam I., Tomita T., Davuluri R.V, & Soares M.B. (2017). Stabilization of HIF-1α and HIF-2α, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS ONE, 12(3), e0173106.

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